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货号： bs-1449R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1449R
- 英文名称
- Prohibitin
- 中文名称
- 抑制素/肿瘤抑制因子抗体
- 别 名
- Mitochondrial Marker; PHB; prohibitin; PHB1; Prohibitin - Mitochondrial Marker; PHB_HUMAN.
- Specific References (2) | bs-1449R has been referenced in 2 publications.[IF=2.08] Xing, Wen Min, et al. "Proteomic identification of mitochondrial targets involved in andrographolide sodium bisulfite-induced nephrotoxicity in a rat model."Environmental Toxicology and Pharmacology (2015). WB ; Rat.PubMed:26356389[IF=5.23] Wang, Jinzi, et al. "Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus." Scientific Reports 6 (2016): 34308. WB ; Other Species.PubMed:27698384
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 染色质和核信号 细胞凋亡 细胞类型标志物
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Horse,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 30kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Prohibitin:171-272/272
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3 UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Function:
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Subunit:
Interacts with PHB2. Interacts with STOML2.
Subcellular Location:
Mitochondrion inner membrane.
Tissue Specificity:
Widely expressed in different tissues.
Similarity:
Belongs to the prohibitin family.
SWISS:
P35232
Gene ID:
5245
Database links:Entrez Gene: 5245 Human
Entrez Gene: 18673 Mouse
Entrez Gene: 25344 Rat
Omim: 176705 Human
SwissProt: P35232 Human
SwissProt: P67778 Mouse
SwissProt: P67779 Rat
Unigene: 514303 Human
Unigene: 263862 Mouse
Unigene: 29754 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Prohibitin(phb)基因是一种有效的肿瘤抑制基因，广泛分布于不同种属的各种生物细胞中，它所编码的产物Prohibitin(PHB)蛋白结构保守，既存在于线粒体内膜上，发挥分子伴侣作用，也存在于细胞核内，具有负性转录调控作用，因而对细胞代谢、生长、分化、衰老以及凋亡等诸多方面发挥 着重要的调控作用，可能与某些肿瘤和退行性疾病的发生有关。
- 产品图片
Sample:
Heart(Mouse) Lysate at 40 ug
Primary: Anti-Prohibitin (bs-1449R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
Sample:
A431(Human) Cell Lysate at 40 ug
Primary: Anti-Prohibitin (bs-1449R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated(bs-1449R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated(bs-1449R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining