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货号: bs-1401R 基本售价: 1380.0 元 规格: 100ul
- 规格:100ul
- 价格:1380.00元
- 规格:200ul
- 价格:2200.00元
产品信息
- 产品编号
- bs-1401R
- 英文名称
- SRF
- 中文名称
- 血清应答因子抗体
- 别 名
- c fos serum response element binding factor; Serum Response Factor SRF; c fos serum response element binding transcription factor; ELK3; ERP; MCM 1; MCM-1; MCM1; OTTHUMP00000039820; SAP2; Serum response factor; SRF; SRF serum response factor c fos serum response element binding transcription factor; SRF_HUMAN.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 干细胞 细胞凋亡 转录调节因子 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 56kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human SRF:101-250/508
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). [provided by RefSeq, Jul 2008].
Function:
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5 of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
Subunit:
Binds DNA as a multimer, probably a dimer. Interacts with MLLT7/FOXO4, NKX3A and SSRP1. Interacts with ARID2 and SRFBP1. Forms complexes with ARID2, MYOCD, NKX2-5 and SRFBP1. Forms a nuclear ternary complex with MKL1 and SCAI.
Subcellular Location:
Nucleus.
Post-translational modifications:
Phosphorylated by PRKDC.
Similarity:
Contains 1 MADS-box domain.
SWISS:
P11831
Gene ID:
6722
Database links:Entrez Gene: 6722 Human
Entrez Gene: 20807 Mouse
Entrez Gene: 501099 Rat
Omim: 600589 Human
SwissProt: P11831 Human
SwissProt: Q9JM73 Mouse
Unigene: 520140 Human
Unigene: 45044 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
转录调节因子(Transcriptin Regulators)
血清应答因子(SRF)是一种转录因子蛋白,可以结合启动子DNA序列中的血清应答元件(SRE),调节一系列基因的表达,包括即刻早期基因的表达,如c-fos、fosB、junB、egr-1、egr-2,以及神经元基因nurr1、肌肉基因如肌动蛋白、肌球蛋白基因等.
- 产品图片
- Sample: NIH/3T3 Cell Lysate at 30 ug
Primary: Anti-SRF (bs-1401R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 56 kD
Observed band size: 54 kDSample: Muscle(Mouse) Lysate at 30 ug
Primary: Anti- SRF (bs-1401R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 56 kD
Observed band size: 56 kDSample: Large intestine (Mouse) Lysate at 30 ug
Primary: Anti- SRF (bs-1401R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 56 kD
Observed band size: 54 kDParaformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SRF) Polyclonal Antibody, Unconjugated (bs-1401R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.