Human Lymphotoxin-alpha/TNF-beta Affinity Purified PAb (100 UG)【科瑞奥博】

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Human Lymphotoxin-alpha/TNF-beta Affinity Purified PAb (100 UG)

货号： AF-211-NA 基本售价：询价规格： -

产品信息

概述
货号	AF-211-NA
别名	LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1
全称	Tumor Necrosis Factor beta
反应种属	Human
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects human Lymphotoxin‑ alpha /TNF‑ beta in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TNF-alpha, rhsTNF RI and rhsTNF RII is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize Lymphotoxin‑ alpha /TNF‑ beta -induced cytotoxicity in the L‑929 mouse fibroblast cell line [Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221]. The Neutralization Dose (ND₅₀) is typically 0.01-0.03 µg/mL in the presence of 0.25 ng/mL Recombinant Human Lymphotoxin‑ alpha /TNF‑ beta and 1 µg/mL actinomycin D.
来源	Polyclonal Goat IgG
产品组分
是否允许进口	否
是否危险品	是

性能
供应商	R&D Systems
Entrez Gene IDs	4049 (Human); 16992 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Lymphotoxin-alphabeta heterotrimers are cleaved by metalloproteinases and contribute to synovitis in rheumatoid arthritis. Authors: Young J, Yu X, Wolslegel K Cytokine, 2010;51(1):78-86. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis. Authors: Casazza S, Zappia E, Pistorio A, Gambini C Proc. Natl. Acad. Sci. U.S.A., 2004;101(30):11064-9. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Lymphotoxin‑ alpha /TNF‑ beta Leu35-Leu205 Accession # P01374
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Tumor necrosis factor beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), and TNF-alpha, are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical, effects. Among these effects is the ability to kill certain tumor cells directly, from which the names tumor necrosis factor and lymphotoxin both derive. Mature TNF-beta /LT-alpha and TNF-alpha share approximately 35% protein sequence homology and the biologically active secreted forms of both proteins are homotrimers. Whereas TNF-alpha can exist as a type II membrane protein, TNF-beta /LT-alpha possesses a typical signal peptide sequence and is a secreted protein. It has been shown that TNF-beta /LT-alpha is also present on the cell surface of activated T, B, and LAK cells as a heteromeric complex with LT-beta, a type II membrane protein that is another member of the TNF ligand family. The genes for TNF-alpha, TNF-beta /LT-alpha, and LT-beta are closely linked within the major histocompatibility complex. TNF-beta /LT-alpha is expressed in activated T- and B-lymphocytes. In addition to its cytotoxic action on tumor cells, TNF-beta /LT-alpha has been shown to be a mediator of inflammation and immune function. Evidence is also accumulating that TNF-beta /LT-alpha and TNF-alpha are mediators in the pathogenesis of certain autoimmune diseases. TNF-beta /LT-alpha has also been shown to have a role in lymphoid organ development. Human and mouse TNF-beta /LT-alpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Co-stimulatory and Co-inhibitory Molecules Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis ICC / IHC Images: Lymphotoxin-alpha/TNF-beta Neuroinflammation Regulation of Apoptosis by TNF Superfamily Members Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members Regulation of T Cell Co-stimulation by TNF Superfamily Members T Cell Cytokine Signaling Th1 Cells TNF Superfamily TNF Superfamily Ligands

参考图片

Cytotoxicity Induced by Lymphotoxin‑ alpha /TNF‑ beta and Neutralization by Human Lymphotoxin‑ alpha /TNF‑ beta Antibody. Recombinant Human Lymphotoxin‑ alpha /TNF‑ beta (Catalog # 211-TB) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Human Lymphotoxin-alpha /TNF-beta (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Lymphotoxin‑ alpha /TNF‑ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-211-NA). The ND₅₀ is typically 0.01‑0.03 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).