TGF-beta Pan Specific Polyclonal Ab (1 MG)【科瑞奥博】

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TGF-beta Pan Specific Polyclonal Ab (1 MG)

货号： AB-100-NA 基本售价： 6107.3 元规格： -

产品信息

概述
货号	AB-100-NA
别名	TGFbeta; TGF-beta
全称	Transforming Growth Factor beta
应用	Western Blot(1 µg/mL)
目标/特异性	Detects TGF-beta 1, TGF-beta 1.2, TGF-beta 2, TGF-beta 3, and TGF-beta 5 in direct ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND₅₀) is approximately 1 µg/mL, 15 µg/mL, 4 µg/mL, and 1 µg/mL for Porcine TGF-beta 1.2, Porcine TGF-beta 2, Recombinant Chicken TGF-beta 3, and Recombinant Amphibian TGF-beta 5, respectively. The ND₅₀ range for Porcine TGF-beta 1 is 5-30 µg/mL.
来源	Polyclonal Rabbit IgG
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TGF-? Neutralization Enhances AngII-Induced Aortic Rupture and Aneurysm in Both Thoracic and Abdominal Regions Authors: X Chen, DL Rateri, DA Howatt, A Balakrishn, JJ Moorleghen, LA Cassis, A Daugherty PLoS ONE, 2016;11(4):e0153811. Species: Mouse Sample Type: In Vivo Application: In vivo Osmotic Induction of Angiogenic Growth Factor Expression in Human Retinal Pigment Epithelial Cells. Authors: Veltmann M, Hollborn M, Reichenbach A, Wiedemann P, Kohen L, Bringmann A PLoS ONE, 2016;11(1):e0147312. Species: Human Sample Type: Whole Cells Application: Neut Lymphatic vessels regulate immune microenvironments in human and murine melanoma J Clin Invest, 2016;0(0):. Species: Human Sample Type: Tissue Homogenates Application: WB Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblasts. Authors: Angelucci C, Maulucci G, Colabianchi A, Iacopino F, DAlessio A, Maiorana A, Palmieri V, Papi M, De Spirito M, Di Leone A, Masetti R, Sica G Br J Cancer, 2015;112(10):1675-86. Species: Human Sample Type: Whole Cells Application: Neut Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment. Authors: Zhang P, Lo A, Huang Y, Huang G, Liang G, Mott J, Karpen G, Blakely E, Bissell M, Barcellos-Hoff M, Snijders A, Mao J Sci Rep, 2015;5(0):8919. Species: Mouse Sample Type: Whole Tissue Application: Blocking CD4(+)Foxp3(+) Tregs protect against innate immune cell-mediated fulminant hepatitis in mice. Authors: Hou X, Song J, Su J, Huang D, Gao W, Yan J, Shen J Mol Immunol, 2015;63(2):420-7. Species: Mouse Sample Type: In Vivo Application: Neut Bmi1 limits dilated cardiomyopathy and heart failure by inhibiting cardiac senescence. Authors: Gonzalez-Valdes I, Hidalgo I, Bujarrabal A, Lara-Pezzi E, Padron-Barthe L, Garcia-Pavia P, Gomez-del Arco P, Redondo J, Ruiz-Cabello J, Jimenez-Borreguero L, Enriquez J, de la Pompa J, Hidalgo A, Gonzalez S Nat Commun, 2015;6(0):6473. Species: Mouse Sample Type: In Vivo Application: In vivo Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation. Authors: Zhu L, Huang Z, Stalesen R, Hansson G, Li N J Thromb Haemost, 2014;12(7):1156-65. Species: Human Sample Type: Whole Cells Application: Bioassay Transglutaminase 2 cross-linking activity is linked to invadopodia formation and cartilage breakdown in arthritis. Authors: Lauzier A, Charbonneau M, Paquette M, Harper K, Dubois CM Arthritis Res. Ther., 2012;14(4):R159. Species: Human Sample Type: Whole Cells Application: Neut Toll-like Receptor 2 (TLR2), Transforming Growth Factor-beta, Hyaluronan (HA), and Receptor for HA-mediated Motility (RHAMM) Are Required for Surfactant Protein A-stimulated Macrophage Chemotaxis. Authors: Foley J, Lam D, Jiang H, Liao J, Cheong N, McDevitt T, Zaman A, Wright J, Savani R J Biol Chem, 2012;287(44):37406-19. Species: Mouse Sample Type: Whole Cells Application: Neut Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade. Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D J Immunol, 2012;189(12):5590-601. Species: Human Sample Type: In Vivo Application: Bioassay Nonmyelinating Schwann cells maintain hematopoietic stem cell hibernation in the bone marrow niche. Authors: Yamazaki S, Ema H, Karlsson G, Yamaguchi T, Miyoshi H, Shioda S, Taketo MM, Karlsson S, Iwama A, Nakauchi H Cell, 2011;147(5):1146-58. Species: Mouse Sample Type: Whole Cells Application: ICC Calpain mediates pulmonary vascular remodeling in rodent models of pulmonary hypertension, and its inhibition attenuates pathologic features of disease. Authors: Ma W, Han W, Greer PA, Tuder RM, Toque HA, Wang KK, Caldwell RW, Su Y J. Clin. Invest., 2011;121(11):4548-66. Species: Mouse Sample Type: In Vivo Application: In vivo IL-33 induces IL-9 production in human CD4+ T cells and basophils. Authors: Blom L, Poulsen BC, Jensen BM, Hansen A, Poulsen LK PLoS ONE, 2011;6(7):e21695. Species: Human Sample Type: Whole Cells Application: Neut The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut. Authors: Suzuki K, Maruya M, Kawamoto S Immunity, 2010;33(1):71-83. Species: Mouse Sample Type: Whole Cells Application: Neut Interferon-gamma and the interferon-inducible chemokine CXCL10 protect against aneurysm formation and rupture. Authors: King VL, Lin AY, Kristo F, Anderson TJ, Ahluwalia N, Hardy GJ, Owens AP, Howatt DA, Shen D, Tager AM, Luster AD, Daugherty A, Gerszten RE Circulation, 2009;119(3):426-35. Species: Mouse Sample Type: In Vivo Application: In vivo Molecular mechanisms of TGFbeta receptor-triggered signaling cascades rapidly induced by the calcineurin inhibitors cyclosporin A and FK506. Authors: Akool el-S, Doller A, Babelova A, Tsalastra W, Moreth K, Schaefer L, Pfeilschifter J, Eberhardt W J. Immunol., 2008;181(4):2831-45. Species: Rat Sample Type: Whole Cells Application: Neut Collagen I promotes epithelial-to-mesenchymal transition in lung cancer cells via transforming growth factor-beta signaling. Authors: Shintani Y, Maeda M, Chaika N, Johnson KR, Wheelock MJ Am. J. Respir. Cell Mol. Biol., 2007;38(1):95-104. Species: Human Sample Type: Whole Cells Application: Neut Advanced glycation end products decrease mesangial cell MMP-7: a role in matrix accumulation in diabetic nephropathy? Authors: McLennan SV, Kelly DJ, Schache M, Waltham M, Dy V, Langham RG, Yue DK, Gilbert RE Kidney Int., 2007;72(4):481-8. Species: Human Sample Type: Whole Cells Application: Neut Transforming growth factor-beta1 suppresses airway hyperresponsiveness in allergic airway disease. Authors: Alcorn JF, Rinaldi LM, Jaffe EF, van Loon M, Bates JH, Janssen-Heininger YM, Irvin CG Am. J. Respir. Crit. Care Med., 2007;176(10):974-82. Species: Mouse Sample Type: In Vivo Application: In vivo Local administration of interleukin-11 ameliorates intestinal radiation injury in rats. Authors: Boerma M, Wang J, Burnett AF, Santin AD, Roman JJ, Hauer-Jensen M Cancer Res., 2007;67(19):9501-6. Species: Rat Sample Type: Whole Cells Application: ICC Increase in transforming growth factor-beta in the brain during infection is related to fever, not depression of spontaneous motor activity. Authors: Matsumura S, Shibakusa T, Fujikawa T, Yamada H, Inoue K, Fushiki T Neuroscience, 2007;144(3):1133-40. Species: Rat Sample Type: CSF Application: Neut Angiotensin II type 1 receptor blockade attenuates TGF-beta-induced failure of muscle regeneration in multiple myopathic states. Authors: Cohn RD, van Erp C, Habashi JP, Soleimani AA, Klein EC, Lisi MT, Gamradt M, ap Rhys CM, Holm TM, Loeys BL, Ramirez F, Judge DP, Ward CW, Dietz HC Nat. Med., 2007;13(2):204-10. Species: Mouse Sample Type: In Vivo Application: In vivo Neuronal TGF-beta1 mediates IL-9/mast cell interaction and exacerbates excitotoxicity in newborn mice. Authors: Mesples B, Fontaine RH, Lelievre V, Launay JM, Gressens P Neurobiol. Dis., 2005;18(1):193-205. Species: Mouse Sample Type: Tissue Homogenates Application: WB Nitric oxide induces TIMP-1 expression by activating the transforming growth factor beta-Smad signaling pathway. Authors: Akool el-S, Doller A, Muller R, Gutwein P, Xin C, Huwiler A, Pfeilschifter J, Eberhardt W J. Biol. Chem., 2005;280(47):39403-16. Species: Rat Sample Type: Whole Cells Application: Neut Uptake of host cell transforming growth factor-beta by Trypanosoma cruzi amastigotes in cardiomyocytes: potential role in parasite cycle completion. Authors: Waghabi MC, Keramidas M, Bailly S, Degrave W, Mendonca-Lima L, Soeiro Mde N, Meirelles Mde N, Paciornik S, Araujo-Jorge TC, Feige JJ Am. J. Pathol., 2005;167(4):993-1003. Species: Mouse Sample Type: Whole Cells Application: Electron Microscopy Thrombospondin plays a vital role in the immune privilege of the eye. Authors: Zamiri P, Masli S, Kitaichi N, Taylor AW, Streilein JW Invest. Ophthalmol. Vis. Sci., 2005;46(3):908-19. Species: Mink Sample Type: Whole Cells Application: Neut Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10. Authors: Zheng SG, Wang JH, Gray JD, Soucier H, Horwitz DA J. Immunol., 2004;172(9):5213-21. Species: Human Sample Type: Whole Cells Application: Neut Effect of weightbearing on bone formation during distraction osteogenesis. Authors: Leung KS, Cheung WH, Yeung HY, Lee KM, Fung KP Clin. Orthop. Relat. Res., 2004;0(419):251-7. Species: Goat Sample Type: Whole Tissue Application: IHC Paraffin-embedded Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates. Authors: Torrealba JR, Katayama M, Fechner JH, Jankowska-Gan E, Kusaka S, Schultz JM, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ J. Immunol., 2004;172(9):5753-64. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Cells Application: Neut Immunoneutralization of growth differentiation factor 9 reveals it partially accounts for mouse oocyte mitogenic activity. Authors: Gilchrist RB, Ritter LJ, Cranfield M, Jeffery LA, Amato F, Scott SJ, Myllymaa S, Kaivo-Oja N, Lankinen H, Mottershead DG, Groome NP, Ritvos O Biol. Reprod., 2004;71(3):732-9. Species: Bovine Sample Type: Whole Cells Application: Neut Connective tissue growth factor and its correlation to other growth factors in experimental granulation tissue. Authors: Inkinen K, Wolff H, Lindroos P, Ahonen J Connect. Tissue Res., 2003;44(1):19-29. Species: Rat Sample Type: Whole Tissue Application: IHC Paraffin-embedded Estrogen inhibition of PTH-stimulated osteoclast formation and attachment in vitro: involvement of both PKA and PKC. Authors: Liu BY, Wu PW, Bringhurst FR, Wang JT Endocrinology, 2002;143(2):627-35. Species: Mouse Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified
免疫原	Recombinant human TGF‑ beta 1, porcine platelet-derived TGF‑ beta 1.2, porcine platelet-derived TGF‑ beta 2, and recombinant amphibian TGF‑ beta 5
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
标记	Unconjugated
溶解方法	Reconstitute at 1 mg/mL in sterile PBS.
背景	TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity. TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription. Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Airway Inflammation and Remodeling B Cell Markers Cytokines and Receptors in Angiogenesis gamma delta T Cells Interactive Pathway: TGF-beta Signaling Pathways Interactive Pathway: Th17 Differentiation Macrophage Activation Markers Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) T Cell Cytokine Signaling TGF-beta Family TGF-beta Family Ligands TGF-beta Signaling Regulates the Expression of Multiple Target Genes with Tumor-Suppressing & Tumor-Promoting Effects TGF-beta-Induced Epithelial-to-Mesenchymal Transition Promotes Breast Cancer Progression Th17 Cells Th22 Cells Th9 Cells

参考图片

TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta Antibody. Porcine TGF-beta 1 (Catalog # 101-B1) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse
T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4
(7.5 ng/mL) activity elicited by Porcine TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of TGF-beta Pan Specific Polyclonal Antibody (Catalog # AB-100-NA). The ND₅₀ range is 5-30 µg/mL.