| 货号 | BAF272 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB272-500；Detection antibody：BAF272; and protein: 272-I-010 |
| 别名 | C-C motif chemokine 1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects human CCL1/I‑309/TCA‑3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with rhMIP-1 beta, rvMIP-1, rrIFN-gamma, rmTCA-3 and rhTARC is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6346 (Human); 20290 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CC chemokine ligand 1 is released into the airways of atopic asthmatics. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CCL1/I‑309/TCA‑3 (R&D Systems, Catalog # 272-I) Lys24-Lys96 Accession # P22362 |
| 生物活性 | Human |
| 标记 | Biotin |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes. CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. The CCL1 sequence contains one potential N-linked glycosylation site and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15-16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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