| 货号 | 32401-1 |
| 别名 | Fibroblast interferon; IFBIFNB; IFF; IFNB1; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956 | 全称 | Interferon beta |
| 反应种属 | Mouse |
| 应用 | Neutralization |
| 目标/特异性 | Detects mouse IFN-beta. |
| 使用方法 | Neutralization: One neutralization unit is the amount of antiserum required to neutralize one unit ofmouse interferon beta (Mu-IFN-beta ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) J. Virol. 37, 755-758] using L929 cells and EMCV as the challenge virus. In this antiviral assay for interferon, about 2.5 unit/ml of interferon is the quantity necessary to produce acytopathic effect of 50%. The units are determined by use of a laboratory standard calibrated with respect to the international reference standard for Mu-IFN-beta provided by the National Institutes of Health [Gb02-902-511]. This materialis prepared specifically for effective neutralization of Mu-IFN-beta. |
| 来源 | Polyclonal Rabbit IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3456 (Human); 15977 (Mouse); 24481 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Histone deacetylase sirtuin 1 deacetylates IRF1 protein and programs dendritic cells to control Th17 protein differentiation during autoimmune inflammation. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Recombinant mouse IFN-beta |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 背景 | Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS. |
| 运输条件 | Dry Ice |
| 存放说明 | -80℃ |
| 参考文献 | Asselin-Paturelet al.(J Exp. Med, 2005) used 32400 in combination with Rabbit anti-mouse IFN-alpha (32100) to explore the role of Type I IFN in the migration and activation of dendritic cells. Kamath et al.(J. Immunol. 2005) used a similar cocktail to demonstrate that dendritic cell derived Type I IFN stimulates bystander T-cells. Seimonet al.(PNAS, 2006) used 32400 to examine the role of IFN Beta in protecting LPS treated macrophages from apoptosis. Zheng et al.(J. Biol. Chem, 2006) used 32400 to examine the role of autocrine IFN Beta in RANKL stimulated iNOS expression in macrophages. |