| 货号 | AB-33-NA |
| 别名 | basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF2; FGF-2; FGF2AS; FGFBprostatropin; fibroblast growth factor 2 (basic); GFG1; HBGF-2; HBGH-2; heparin-binding growth factor 2; NUDT6; Prostatropin | 全称 | Fibroblast Growth Factor basic |
| 反应种属 | Bovine |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects bovine FGF basic in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with FGF acidic and recombinant human (rh) beta -ECGF is observed and less than 1% cross-reactivity with rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7, recombinant mouse (rm) FGF-8b, rmFGF-8c, rhFGF-9, rhFGF-10, rmFGF-15, rhFGF-17, and rhFGF-18 is observed. Neutralizes the biological activity of bovine FGF basic and will also neutralize the biological activity of recombinant human FGF basic. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize FGF basic-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 1.5-4.5 µg/mL in the presence of 0.5 ng/mL Bovine FGF basic. |
| 来源 | Polyclonal Rabbit IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Maternal obesity and overnutrition alter fetal growth rate and cotyledonary vascularity and angiogenic factor expression in the ewe. | |
| 纯化方式 | Protein A or G purified |
| 免疫原 | Bovine brain-derived FGF acidic |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Bovine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 1 mg/mL in sterile PBS. |
| 背景 | FGF basic is a member of the FGF family, currently comprised of seven related mitogenic proteins which show 35‑55% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum and placenta. This factor contains four cysteine residues but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. Several reports indicate that a variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions apparently affect localization of FGF basic in cellular compartments but do not affect biological activity. Studies indicate that binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin, and many cells of neuroectodermal, ectodermal and endodermal origin. The cells include fibroblasts, endothelial cells, astrocytes, oligodendrocytes, neuroblasts, keratinocytes, osteoblasts, smooth muscle cells, and melanocytes. FGF basic is chemotactic and mitogenic for endothelial cells in vitro. FGF basic induces neuron differentiation, survival and regeneration. FGF basic has also been shown to be crucial in modulating embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Cell Proliferation Induced by FGF basic and Neutralization by Bovine FGF basic Antibody. Bovine FGF basic (Catalog # 133-FB) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF basic (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Bovine FGF basic Polyclonal Antibody (Catalog # AB-33-NA). The ND50 is typically 1.5-4.5 µg/mL. |
