Bovine FGF acidic Polyclonal Ab (Rabbit) (1 MG)【科瑞奥博】

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Bovine FGF acidic Polyclonal Ab (Rabbit) (1 MG)

货号： AB-32-NA 基本售价： 5552.1 元规格： -

产品信息

概述
货号	AB-32-NA
别名	AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF1; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1
全称	Fibroblast Growth Factor acidic
反应种属	Bovine
应用	Western Blot(1 µg/mL)
目标/特异性	Detects bovine FGF acidic in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody will recognize both bovine FGF acidic and recombinant human FGF acidic. In direct ELISAs, no cross‑reactivity with __________________ is observed. Neutralizes the biological activity of bovine FGF acidic and will also neutralize the biological activity of rhFGF acidic, although 5 times the amount of IgG is required.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize FGF acidic-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 1-3 µg/mL in the presence of 0.75 ng/mL Bovine FGF acidic.
来源	Polyclonal Rabbit IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Human FGF-1 gene transfer promotes the formation of collateral vessels and arterioles in ischemic muscles of hypercholesterolemic hamsters. Authors: Caron A, Michelet S, Caron A, Sordello S, Ivanov MA, Delaere P, Branellec D, Schwartz B, Emmanuel F J Gene Med, 2004;6(9):1033-45. Species: Hamster Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Protein A or G purified
免疫原	Bovine brain-derived FGF acidic
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Bovine
标记	Unconjugated
溶解方法	Reconstitute at 1 mg/mL in sterile PBS.
背景	FGF acidic, also known as FGF-1, ECGF, and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF acidic is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF acidic can translocate to the cytosol and to the nucleus. Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Gimenez-Gallego, G. et al. (1985) Science 230:1385. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159. Rajalingam, D. et al. (2007) Biochemistry 46:9225. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637. Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45. Wesche, J. et al. (2006) J. Biol. Chem. 281:11405. Imamura, T. et al. (1990) Science 249:1567. Wesche, J. et al. (2005) Biochemistry 44:6071. Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794. Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245. Bouleau, S. et al. (2005) Oncogene 24:7839.

参考图片

Cell Proliferation Induced by FGF acidic and Neutralization by Bovine FGF acidic Antibody. Bovine FGF acidic (Catalog # 132‑FA) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF acidic (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti‑Bovine FGF acidic Polyclonal Antibody (Catalog # AB‑32‑NA). The ND₅₀ is typically 1‑3 µg/mL.