Human MMP-8 Biotinylated Affinity Purified PAb (50 UG)【科瑞奥博】

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Human MMP-8 Biotinylated Affinity Purified PAb (50 UG)

货号： BAF908 基本售价： 5532.3 元规格： -

产品信息

概述
货号	BAF908
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB908；Detection antibody：BAF908; and protein: 908-MP-010
别名	CLG1HNC; Collagenase 2; EC 3.4.24; EC 3.4.24.34; matrix metallopeptidase 8 (neutrophil collagenase); matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP-8; neutrophil collagenase; PMNL collagenase; PMNL-CL
全称	Matrix Metalloproteinase 8
反应种属	Human
应用	Western Blot,Immunohistochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard
目标/特异性	Detects human MMP‑8 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) rhMMP-1, rhMMP-2, rhMMP-9, rhMMP-13, rhMMP-7, rhMMP-10 and rhMMP-12 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard :
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4317 (Human); 17394 (Mouse); 63849 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human MMP‑8 Phe21-Gly467 Accession # AAZ38714
生物活性	Human
标记	Biotin
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta -casein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis, MMP-8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP‑8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexin-like domain. MMP-8 is heavily glycosylated.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Cancer Biomarkers Collagen Modifying Enzymes ICC / IHC Images: MMP-8 Metalloproteases and Regulators MMP Modulation of Cytokines MMP-12 in Smoking-Related Pulmonary Disease MMPs, TIMPs and Related Molecules New Tools: Active MMP Activity Assays OSM Induction of Chondrocyte MMP and TIMP-3 Gene Expression

参考图片

MMP‑8 in Human Breast Cancer Tissue. MMP‑8 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human
MMP‑8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF908) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in ductal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.