Rat IL-1 beta/IL-1F2 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Rat IL-1 beta/IL-1F2 Affinity Purified Polyclonal Ab (100 UG)

货号： AF-501-NA 基本售价： 5135.7 元规格： -

产品信息

概述
货号	AF-501-NA
描述	For ELISA the Antibody Pairs information：Capture antibody：AF-501-NA；Detection antibody：BAF501; and protein: 501-RL-010
别名	catabolin; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
全称	Interleukin 1 beta
反应种属	Rat
应用	Western Blot,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects rat IL-1 beta /IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant mouse IL‑1 beta is observed and less than 0.5% cross-reactivity with recombinant human IL-1 beta and recombinant porcine IL-1 beta is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 2-10 µg/mL in the presence of 10 ng/mL Recombinant Rat IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Neuroinflammation increases GABAergic tone and impairs cognitive and motor function in hyperammonemia by increasing GAT-3 membrane expression. Reversal by sulforaphane by promoting M2 polarization of microglia Authors: V Hernandez-, A Cabrera-Pa, L Taoro-Gonz, A Gonzalez-U, A Agusti, T Balzano, M Llansola, V Felipo J Neuroinflammation, 2016;13(1):83. In Vitro Neuroprotective and Anti-Inflammatory Activities of Natural and Semi-Synthetic Spirosteroid Analogues Molecules, 2016;21(8):. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Spatiotemporal Cadence of Macrophage Polarisation in a Model of Light-Induced Retinal Degeneration. Authors: Jiao H, Natoli R, Valter K, Provis J, Rutar M PLoS ONE, 2015;10(12):e0143952. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Spinal cord injury induces a long-lasting upregulation of interleukin-1beta in astrocytes around the central canal. Authors: Paniagua-Torija B, Arevalo-Martin A, Molina-Holgado E, Molina-Holgado F, Garcia-Ovejero D Neuroscience, 2015;284(0):283-9. Species: Rat Sample Type: tissue Application: IHC Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats. Authors: Fu H, Yang T, Xiao W, Fan L, Wu Y, Terrando N, Wang T PLoS ONE, 2014;9(8):e106331. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone. Authors: Shigemoto-Mogami Y, Hoshikawa K, Goldman J, Sekino Y, Sato K J Neurosci, 2014;34(6):2231-43. Species: Rat Sample Type: Whole Cells Application: Neut Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain. J Neuroinflammation, 2012;9(0):181. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Unmasking of LPA1 receptor-mediated migration response to lysophosphatidic acid by interleukin-1beta-induced attenuation of Rho signaling pathways in rat astrocytes. Authors: Sato K, Horiuchi Y, Jin Y, Malchinkhuu E, Komachi M, Kondo T, Okajima F J. Neurochem., 2011;117(1):164-74. Species: Rat Sample Type: Cell Lysate Application: WB Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia. Authors: Qu WS, Wang YH, Ma JF, Tian DS, Zhang Q, Pan DJ, Yu ZY, Xie MJ, Wang JP, Wang W J. Neurochem., 2011;116(2):217-26. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Microglial cells contribute to endogenous brain defenses after acute neonatal focal stroke. Authors: Faustino JV, Wang X, Johnson CE J. Neurosci., 2011;31(36):12992-3001. Species: Rat Sample Type: Whole Tissue Application: IHC Peripheral osmotic stimulation inhibits the brains innate immune response to microdialysis of acidic perfusion fluid adjacent to supraoptic nucleus. Authors: Summy-Long JY, Hu S Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009;297(5):R1532-45. Species: Rat Sample Type: Whole Tissue Application: IHC Spinal leptin contributes to the pathogenesis of neuropathic pain in rodents. Authors: Lim G, Wang S, Zhang Y, Tian Y, Mao J J. Clin. Invest., 2009;119(2):295-304. Species: Rat Sample Type: Tissue Homogenates Application: WB Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology. Authors: Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA Infect. Immun., 2008;76(8):3577-86. Species: Rat Sample Type: BALF Application: ELISA Development Systemic immune challenge activates an intrinsically regulated local inflammatory circuit in the adrenal gland. Authors: Engstrom L, Rosen K, Angel A, Fyrberg A, Mackerlova L, Konsman JP, Engblom D, Blomqvist A Endocrinology, 2008;149(4):1436-50. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen IL-1beta promotes neurite outgrowth by deactivating RhoA via p38 MAPK pathway. Authors: Temporin K, Tanaka H, Kuroda Y, Okada K, Yachi K, Moritomo H, Murase T, Yoshikawa H Biochem. Biophys. Res. Commun., 2007;365(2):375-80. Species: Rat Sample Type: Whole Cells Application: ICC Wnt-induced secreted protein-1 is a prohypertrophic and profibrotic growth factor. Authors: Colston JT, de la Rosa SD, Koehler M, Gonzales K, Mestril R, Freeman GL, Bailey SR, Chandrasekar B Am. J. Physiol. Heart Circ. Physiol., 2007;293(3):H1839-46. Species: Rat Sample Type: Whole Cells Application: Neut Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation. Authors: Barkho BZ, Song H, Aimone JB Stem Cells Dev., 2006;15(3):407-21. Species: Rat Sample Type: Whole Cells Application: Neut In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids. Authors: Roche M, Diamond M, Finn DP J. Neuroimmunol., 2006;181(1):57-67. Species: Rat Sample Type: Plasma Application: ELISA Development Single subcutaneous administration of chorionic gonadotropin to rats induces a rapid and transient increase in testicular expression of pro-inflammatory cytokines. Authors: Assmus M, Svechnikov K, von Euler M, Setchell B, Sultana T, Zetterstrom C, Holst M, Soder O Pediatr. Res., 2005;57(6):896-901. Species: Rat Sample Type: Whole Tissue Application: IHC Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression. Authors: Park EM, Cho BP, Volpe BT, Cruz MO, Joh TH, Cho S Neuroscience, 2005;132(3):625-31. Species: Rat Sample Type: Cell Lysates Application: WB A dual role for apolipoprotein e in neuroinflammation: anti- and pro-inflammatory activity. Authors: Guo L, LaDu MJ, Van Eldik LJ J. Mol. Neurosci., 2004;23(3):205-12. Species: Rat Sample Type: Cell Lysates Application: WB Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats. Authors: Nedrebo T, Reed RK, Jonsson R, Berg A, Wiig H J. Physiol. (Lond.), 2004;556(0):193-202. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Glucocorticoids play a fundamental role in protecting the brain during innate immune response. Authors: Nadeau S, Rivest S J. Neurosci., 2003;23(13):5536-44. Species: Rat Sample Type: In Vivo Application: In vivo
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant rat IL-1 beta /IL-1F2 (R&D Systems, Catalog # 501-RL) Val117-Ser268 Accession # Q63264
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The rat IL-1 beta cDNA encodes a 268 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 65-77% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Cancer Biomarkers Chronic IL-1 beta-induced Neuroinflammation Is it Really that Bad? Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis ICC / IHC Images: IL-1 beta/IL-1F2 IL-1 Family Infectious Diseases Inflammasomes: Intracellular Regulators of Pathogen Inflammatory Mediators Interactive Pathway: IL-1 Family Signaling Pathways Isolation, Culture, and Identification of Natural Killer Cells LTi Cells Macrophage Activation Markers Mini-review: Interleukin 1 Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) NCR-ILC3 Neuroinflammation NOD-like Receptors and the Inflammasome Platelets Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th17 Cells Th9 Cells

参考图片

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Rat IL‑1 beta /IL‑1F2 Antibody. Recombinant Rat IL‑1 beta /IL‑1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑1 beta /IL‑1F2 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA). The ND₅₀ is typically 2-10 µg/mL in the presence of concanavalin A (1.25 µg/mL).

IL‑1 beta /IL‑1F2 in Rat Splenocytes. IL‑1 beta /IL‑1F2 was detected in immersion fixed LPS-stimulated rat splenocytes using 5 µg/mL Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑501‑NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.