Mouse CXCL9/MIG Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Mouse CXCL9/MIG Affinity Purified Polyclonal Ab (100 UG)

货号： AF-492-NA 基本售价： 5135.7 元规格： -

产品信息

概述
货号	AF-492-NA
描述	For ELISA the Antibody Pairs information：Capture antibody：AF-492-NA；Detection antibody：BAF492; and protein: 492-MM-010
别名	chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma
反应种属	Mouse
应用	Western Blot(0.5 µg/mL) ELISA Capture (Matched Antibody Pair)(0.2-0.8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects mouse CXCL9/MIG in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) CXCL9/MIG is observed and less than 0.005% cross-reactivity with recombinant mouse MIP-2, recombinant rat CINC-1, rhGRO alpha, rhGRO beta, and rhGRO gamma is observed.
使用方法	Western Blot: 0.5 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CXCL9/MIG-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR3. The Neutralization Dose (ND₅₀) is typically 6-20 µg/mL in the presence of 1 µg/mL Recombinant Mouse CXCL9/MIG.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4283 (Human); 17329 (Mouse); 246759 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata J Immunol, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: Flow Tumor cell-activated CARD9 signaling contributes to metastasis-associated macrophage polarization. Authors: Yang M, Shao J, Miao Y, Cui W, Qi Y, Han J, Lin X, Du J Cell Death Differ, 2014;21(8):1290-302. Species: Mouse Sample Type: Whole Cells Application: Neut Trafficking of high avidity HER-2/neu-specific T cells into HER-2/neu-expressing tumors after depletion of effector/memory-like regulatory T cells. Authors: Weiss VL, Lee TH, Song H, Kouo TS, Black CM, Sgouros G, Jaffee EM, Armstrong TD PLoS ONE, 2012;7(2):e31962. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded A novel combination immunotherapy for cancer by IL-13Ralpha2-targeted DNA vaccine and immunotoxin in murine tumor models. Authors: Nakashima H, Terabe M, Berzofsky JA, Husain SR, Puri RK J. Immunol., 2011;187(10):4935-46. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow Monokine induced by interferon-gamma (MIG/CXCL9) is derived from both donor and recipient sources during rejection of class II major histocompatibility complex disparate skin allografts. Authors: Auerbach MB, Shimoda N, Amano H, Rosenblum JM, Kish DD, Farber JM, Fairchild RL Am. J. Pathol., 2009;174(6):2172-81. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen L-selectin-negative CCR7- effector and memory CD8+ T cells enter reactive lymph nodes and kill dendritic cells. Authors: Guarda G, Hons M, Soriano SF, Huang AY, Polley R, Martin-Fontecha A, Stein JV, Germain RN, Lanzavecchia A, Sallusto F Nat. Immunol., 2007;8(7):743-52. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Viral infection of the lungs through the eye. Authors: Bitko V, Musiyenko A, Barik S J. Virol., 2007;81(2):783-90. Species: Mouse Sample Type: Tissue Homogenates Application: WB The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells. Authors: Fujita H, Asahina A, Komine M, Tamaki K Cell. Immunol., 2007;245(2):70-9. Species: Mouse Sample Type: Whole Cells Application: Neut IL-21 induces tumor rejection by specific CTL and IFN-gamma-dependent CXC chemokines in syngeneic mice. Authors: Di Carlo E, Comes A, Orengo AM, Rosso O, Meazza R, Musiani P, Colombo MP, Ferrini S J. Immunol., 2004;172(3):1540-7. Species: Mouse Sample Type: Whole Tissue Application: IHC Fresh/Frozen Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment. Authors: Janatpour MJ, Hudak S, Sathe M, McEvoy LM J. Exp. Med., 2001;194(9):1375-84. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CXCL9/MIG Accession # P18340
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CXCL9, also known as MIG, is a member of the alpha subfamily of chemokines that lacks the ELR domain, and was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9was subsequently cloned using mouse CXCL9 cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The mouse CXCL9 cDNA encodes a 126 amino acid residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield a 105 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Cancer Biomarkers Chemokines and Receptors Chemokines and Receptors in Angiogenesis CXC Chemokines and Receptors ICC / IHC Images: CXCL9/MIG Inflammatory Mediators Macrophage Activation Markers Regulatory T Cells (Tregs)

参考图片

Detection of Mouse CXCL9/MIG by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-492-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL9/MIG at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chemotaxis Induced by CXCL9/MIG and Neutralization by Mouse CXCL9/MIG Antibody. Recombinant Mouse CXCL9/MIG (Catalog #
492-MM) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL9/MIG (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-492-NA). The ND₅₀ is typically 6-20 µg/mL.