Mouse CCL2/JE/MCP-1 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Mouse CCL2/JE/MCP-1 Affinity Purified Polyclonal Ab (100 UG)

货号： AF-479-NA 基本售价： 5135.7 元规格： -

产品信息

概述
货号	AF-479-NA
描述	For ELISA the Antibody Pairs information：Capture antibody：AF-479-NA；Detection antibody：BAF479; and protein: 479-JE-010
别名	MCAF; MCP-1
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) ELISA Capture (Matched Antibody Pair)(0.2-0.8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects mouse CCL2/JE/MCP-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.01% cross-reactivity with recombinant human (rh) MCP‑1, rhMCP‑2, rhMCP-3, recombinant mouse (rm) MARC, rhMCP-4, rmMCP-5, rmMDC, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-1 gamma, rmMIP‑2, rmMIP‑3 beta, rmRANTES, rmTARC, and rmTeck is observed.
使用方法	Western Blot: 0.1 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND₅₀) is typically 0.5-2.5 µg/mL in the presence of 0.04 µg/mL Recombinant Mouse CCL2/JE/MCP‑1.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The Chemokine CCL2 Mediates the Seizure-enhancing Effects of Systemic Inflammation Authors: C Cerri, S Genovesi, M Allegra, F Pistillo, U Püntener, A Guglielmot, VH Perry, Y Bozzi, M Caleo J Neurosci, 2016;36(13):3777-88. Species: Mouse Sample Type: In Vivo Application: In vivo GEC-derived SFRP5 inhibits Wnt5a-induced macrophage chemotaxis and activation. Authors: Zhao C, Bu X, Wang W, Ma T, Ma H PLoS ONE, 2014;9(1):e85058. Species: Human Sample Type: Whole Cells Application: Neut Persistent inflammation subverts thrombospondin-1-induced regulation of retinal angiogenesis and is driven by CCR2 ligation. Authors: Chen M, Copland DA, Zhao J, Liu J, Forrester JV, Dick AD, Xu H Am. J. Pathol., 2012;180(1):235-45. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development Toll-like receptor 2 contributes to chemokine gene expression and macrophage infiltration in the dorsal root ganglia after peripheral nerve injury. Authors: Kim D, You B, Lim H, Lee SJ Mol Pain, 2011;7(0):74. Species: Mouse Sample Type: Whole Cells Application: ICC Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells. Authors: Puddu P, Latorre D, Carollo M, Catizone A, Ricci G, Valenti P, Gessani S PLoS ONE, 2011;6(7):e22504. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP J. Immunol., 2009;183(9):5799-806. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Stromal MCP-1 in mammary tumors induces tumor-associated macrophage infiltration and contributes to tumor progression. Authors: Fujimoto H, Sangai T, Ishii G, Ikehara A, Nagashima T, Miyazaki M, Ochiai A Int. J. Cancer, 2009;125(6):1276-84. Species: Mouse Sample Type: In Vivo Application: In vivo Reversal of axonal loss and disability in a mouse model of progressive multiple sclerosis. Authors: Basso AS, Frenkel D, Quintana FJ, Costa-Pinto FA, Petrovic-Stojkovic S, Puckett L, Monsonego A, Bar-Shir A, Engel Y, Gozin M, Weiner HL J. Clin. Invest., 2008;118(4):1532-43. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes. Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y J. Neurosci., 2008;28(13):3277-90. Species: Mouse Sample Type: Whole Cells Application: ICC Thrombin induces fibroblast CCL2/JE production and release via coupling of PAR1 to Galphaq and cooperation between ERK1/2 and Rho kinase signaling pathways. Authors: Deng X, Mercer PF, Scotton CJ, Gilchrist A, Chambers RC Mol. Biol. Cell, 2008;19(6):2520-33. Species: Mouse Sample Type: Whole Cells Application: ICC CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T Immunology, 2008;125(2):272-9. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Megalin contributes to the early injury of proximal tubule cells during nonselective proteinuria. Authors: Motoyoshi Y, Matsusaka T, Saito A, Pastan I, Willnow TE, Mizutani S, Ichikawa I Kidney Int., 2008;74(10):1262-9. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection. Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A J. Exp. Med., 2008;205(9):2075-84. Species: Mouse Sample Type: In Vivo Application: In vivo Vascular and inflammatory stresses mediate atherosclerosis via RAGE and its ligands in apoE-/- mice. Authors: Harja E, Bu DX, Hudson BI, Chang JS, Shen X, Hallam K, Kalea AZ, Lu Y, Rosario RH, Oruganti S, Nikolla Z, Belov D, Lalla E, Ramasamy R, Yan SF, Schmidt AM J. Clin. Invest., 2008;118(1):183-94. Species: Mouse Sample Type: Cell Lysates Application: WB TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus. Authors: Zhao Z, Burkly LC, Campbell S, Schwartz N, Molano A, Choudhury A, Eisenberg RA, Michaelson JS, Putterman C J. Immunol., 2007;179(11):7949-58. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Chemical interactions between fibrosarcoma cancer cells and sensory neurons contribute to cancer pain. Authors: Khasabova IA, Stucky CL, Harding-Rose C, Eikmeier L, Beitz AJ, Coicou LG, Hanson AE, Simone DA, Seybold VS J. Neurosci., 2007;27(38):10289-98. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo. Authors: Martin AP, Canasto-Chibuque C, Shang L, Rollins BJ, Lira SA J. Immunol., 2006;177(10):7296-302. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury. Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA J. Immunol., 2006;177(1):612-20. Species: Human Sample Type: BALF Application: ELISA Development An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG J. Exp. Med., 2006;203(12):2763-77. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen CCL2 regulates angiogenesis via activation of Ets-1 transcription factor. Authors: Stamatovic SM, Keep RF, Mostarica-Stojkovic M, Andjelkovic AV J. Immunol., 2006;177(4):2651-61. Species: Mouse Sample Type: Whole Cells Application: Neut Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG J. Immunol., 2006;177(3):1967-74. Species: Mouse Sample Type: Plasma Application: ELISA Development Antibody-bound beta-amyloid precursor protein stimulates the production of tumor necrosis factor-alpha and monocyte chemoattractant protein-1 by cortical neurons. Authors: Gonzalez de Aguilar JL, Dupuis L, Larmet Y Neurobiol. Dis., 2005;19(1):129-41. Species: Mouse Sample Type: Cell Lysates Application: WB Sequential ELISA to profile multiple cytokines from small volumes. Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG J. Immunol. Methods, 2005;302(1):172-81. Species: Mouse Sample Type: Plasma Application: ELISA Development Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration. Authors: Perrin FE, Lacroix S, Aviles-Trigueros M, David S Brain, 2005;128(0):854-66. Species: Mouse Sample Type: In Vivo Application: In vivo Selective macrophage suppression during sepsis. Authors: Ellaban E, Bolgos G, Remick D Cell. Immunol., 2004;231(1):103-11. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells. Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA Blood, 2004;104(5):1404-10. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development A critical role for CCR2/MCP-1 interactions in the development of idiopathic pneumonia syndrome after allogeneic bone marrow transplantation. Authors: Hildebrandt GC, Duffner UA, Olkiewicz KM, Corrion LA, Willmarth NE, Williams DL, Clouthier SG, Hogaboam CM, Reddy PR, Moore BB, Kuziel WA, Liu C, Yanik G, Cooke KR Blood, 2004;103(6):2417-26. Species: Mouse Sample Type: Whole Tissue Application: IHC Possible co-regulation of genes associated with enhanced progression of mammary adenocarcinomas. Authors: Neumark E, Cohn MA, Lukanidin E, Witz IP, Ben-Baruch A Immunol. Lett., 2002;82(1):111-21. Species: Mouse Sample Type: Cell Culture Supernates Application: DB The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium. Authors: Huo Y, Weber C, Forlow SB, Sperandio M, Thatte J, Mack M, Jung S, Littman DR, Ley K J. Clin. Invest., 2001;108(9):1307-14. Species: Mouse Sample Type: Whole Cells Application: Neut Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats. Authors: Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA J. Immunol., 2000;164(5):2650-9. Species: Rat Sample Type: BALF Application: ELISA Development Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function. Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C Immunity, 0;43(5):945-58. Species: Mouse Sample Type: Tissue HomogenatesLung Application: ELISA Capture
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CCL2/JE/MCP-1 Gln24-Arg96 Accession # Q5SVU3
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.
运输条件	Blue Ice
存放说明	-20℃
参考文献	CC Chemokines and Receptors Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis gamma delta T Cells HIF Transcription Factors ICC / IHC Images: CCL2/JE/MCP-1 Inflammatory Mediators Macrophage Activation Markers Neuroinflammation

参考图片

Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody. Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL2/JE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA). The ND₅₀ is typically 0.5-2.5 µg/mL.