Mouse CXCL2/MIP-2 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Mouse CXCL2/MIP-2 Affinity Purified Polyclonal Ab (100 UG)

货号： AF-452-NA 基本售价： 5135.7 元规格： -

产品信息

概述
货号	AF-452-NA
别名	chemokine (C-X-C motif) ligand 2; CINC-2a; CINC-3; C-X-C Motif Chemokine 2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse CXCL2/MIP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) GRO alpha, rhGRO beta, rhGRO gamma, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, and rrCINC-3 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize CXCL2/MIP-2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.015-0.075 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/MIP-2.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2920 (Human); 20310 (Mouse); 114105 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy. Authors: Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K J Biol Chem, 2015;290(4):2053-68. Species: Mouse Sample Type: Whole Tissue Application: IHC The HMGB1/RAGE axis triggers neutrophil-mediated injury amplification following necrosis. Authors: Huebener, Peter, Pradere, Jean-Phi, Hernandez, Celine, Gwak, Geum-You, Caviglia, Jorge Ma, Mu, Xueru, Loike, John D, Jenkins, Rosalind, Antoine, Daniel J, Schwabe, Robert F J Clin Invest, 2015;125(2):539-50. Species: Mouse Sample Type: Whole Cells Application: Neut CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta. Authors: Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S Int J Cancer, 2014;134(6):1346-58. Type I IFN suppresses Cxcr2 driven neutrophil recruitment into the sensory ganglia during viral infection. Authors: Stock A, Smith J, Carbone F J Exp Med, 2014;211(5):751-9. Species: Mouse Sample Type: Whole Cells Application: ICC Neutrophil dependence of vascular remodeling after Mycoplasma infection of mouse airways. Authors: Baluk P, Phillips K, Yao L, Adams A, Nitschke M, McDonald D Am J Pathol, 2014;184(6):1877-89. Species: Mouse Sample Type: Whole Tissue Application: IHC Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow Initial delay in the immune response to Francisella tularensis is followed by hypercytokinemia characteristic of severe sepsis and correlating with upregulation and release of damage-associated molecular patterns. Authors: Mares CA, Ojeda SS, Morris EG, Li Q, Teale JM Infect. Immun., 2008;76(7):3001-10. Species: Mouse Sample Type: Whole Cells Application: Functional Assay The Duffy antigen receptor for chemokines in acute renal failure: A facilitator of renal chemokine presentation. Authors: Zarbock A, Schmolke M, Bockhorn SG, Scharte M, Buschmann K, Ley K, Singbartl K Crit. Care Med., 2007;35(9):2156-63. Species: Mouse Sample Type: In Vivo Application: In vivo CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response. Authors: Cardona AE, Gonzalez PA, Teale JM Infect. Immun., 2003;71(5):2634-42. Species: Mouse Sample Type: Whole Cells Application: ICC The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium. Authors: Huo Y, Weber C, Forlow SB, Sperandio M, Thatte J, Mack M, Jung S, Littman DR, Ley K J. Clin. Invest., 2001;108(9):1307-14. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CXCL2/MIP-2 Ala28-Asn100 Accession # P10889
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines. MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs. Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Chemokines and Receptors in Angiogenesis CXC Chemokines and Receptors ICC / IHC Images: CXCL2/GRO beta/MIP-2/CINC-3 Inflammatory Mediators Macrophage Activation Markers Neuroinflammation

参考图片

Chemotaxis Induced by CXCL2/MIP-2 and Neutralization by Mouse CXCL2/
MIP-2 Antibody. Recombinant Mouse CXCL2/MIP-2 (Catalog # 452‑M2) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL2/MIP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA). The ND₅₀ is typically 0.015‑0.075 µg/mL.

CXCL2/MIP‑2 in Mouse Splenocytes. CXCL2/MIP‑2 was detected in immersion fixed mouse splenocytes stimulated with LPS and monensin using Goat Anti-Mouse CXCL2/MIP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.