Mouse CCL11/Eotaxin Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Mouse CCL11/Eotaxin Affinity Purified Polyclonal Ab (100 UG)

货号： AF-420-NA 基本售价： 5135.7 元规格： -

产品信息

概述
货号	AF-420-NA
描述	For ELISA the Antibody Pairs information：Capture antibody：AF-420-NA；Detection antibody：BAF420; and protein: 420-ME-020
别名	C-C motif chemokine 11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
反应种属	Mouse
应用	Western Blot,Immunohistochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects mouse CCL11/Eotaxin in ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human CCL11/Eotaxin and recombinant mouse (rm) MARC is observed, and less than 2% cross-reactivity with rmJE is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL11/Eotaxin-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND₅₀) is typically 0.1-0.5 µg/mL in the presence of 10 ng/mL Recombinant Mouse CCL11/Eotaxin.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6356 (Human); 20292 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development The ageing systemic milieu negatively regulates neurogenesis and cognitive function. Authors: Villeda SA, Luo J, Mosher KI, Zou B, Britschgi M, Bieri G, Stan TM, Fainberg N, Ding Z, Eggel A, Lucin KM, Czirr E, Park JS, Couillard-Despres S, Aigner L, Li G, Peskind ER, Kaye JA, Quinn JF, Galasko DR, Xie XS, Rando TA, Wyss-Coray T Nature, 2011;477(7362):90-4. Species: Mouse Sample Type: Whole Cells Application: Neut House dust mite induces direct airway inflammation in vivo: implications for future disease therapy? Authors: De Alba J, Raemdonck K, Dekkak A, Collins M, Wong S, Nials AT, Knowles RG, Belvisi MG, Birrell MA Eur. Respir. J., 2010;35(6):1377-87. Species: Rat Sample Type: Whole Tissue Application: IHC Paraffin-embedded Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow CCR3 is a target for age-related macular degeneration diagnosis and therapy. Authors: Takeda A, Baffi JZ, Kleinman ME, Cho WG, Nozaki M, Yamada K, Kaneko H, Albuquerque RJ, Dridi S, Saito K, Raisler BJ, Budd SJ, Geisen P, Munitz A, Ambati BK, Green MG, Ishibashi T, Wright JD, Humbles AA, Gerard CJ, Ogura Y, Pan Y, Smith JR, Grisanti S, Hartnett ME, Rothenberg ME, Ambati J Nature, 2009;460(7252):225-30. Species: Mouse Sample Type: Vitreous Humor Application: Neut Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway. Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM Am. J. Respir. Crit. Care Med., 2009;179(9):772-81. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development IL-13Ralpha2 and IL-10 coordinately suppress airway inflammation, airway-hyperreactivity, and fibrosis in mice. Authors: Wilson MS, Elnekave E, Mentink-Kane MM, Hodges MG, Pesce JT, Ramalingam TR, Thompson RW, Kamanaka M, Flavell RA, Keane-Myers A, Cheever AW, Wynn TA J. Clin. Invest., 2007;117(10):2941-51. Species: Mouse Sample Type: Serum Toll-like receptor-2-mediated C-C chemokine receptor 3 and eotaxin-driven eosinophil influx induced by Mycobacterium bovis BCG pleurisy. Authors: D&amp;apos;Avila H, Almeida PE, Roque NR, Castro-Faria-Neto HC, Bozza PT Infect. Immun., 2007;75(3):1507-11. Species: Mouse Sample Type: In Vivo Application: In vivo Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG J. Immunol., 2006;177(3):1967-74. Species: Mouse Sample Type: Plasma Application: ELISA Development Sequential ELISA to profile multiple cytokines from small volumes. Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG J. Immunol. Methods, 2005;302(1):172-81. Species: Mouse Sample Type: Plasma Application: ELISA Development A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection. Authors: Reading PC, Symons JA, Smith GL J. Immunol., 2003;170(3):1435-42. Species: Human Sample Type: BALF Application: ELISA Development P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses. Authors: Larbi KY, Dangerfield JP, Culley FJ, Marshall D, Haskard DO, Jose PJ, Williams TJ, Nourshargh S J. Leukoc. Biol., 2003;73(1):65-73. Species: Mouse Sample Type: Peritoneal Fluid Application: ELISA Development AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity. Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ Am. J. Pathol., 2002;160(4):1353-60. Species: Human Sample Type: Tissue Homogenates Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CCL11/Eotaxin His24-Pro97 Accession # P48298
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Mouse CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high amino acid sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma. In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Rothenberg, M.E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8960. Kitaura, M. et al. (1996) J. Biol. Chem 271:7725. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.

参考图片

Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Mouse CCL11/
Eotaxin Antibody.
Recombinant Mouse CCL11/
Eotaxin (Catalog # 420‑ME) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL11/
Eotaxin (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA). The ND₅₀ is typically
0.1‑0.5 µg/mL.

CCL11/Eotaxin in Mouse Thymus. CCL11/Eotaxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

CCL11/Eotaxin in Mouse Colon. CCL11/Eotaxin was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse CCL11/Eotaxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-420-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.