Human MMP-1 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human MMP-1 Affinity Purified Polyclonal Ab (100 UG)

货号： AF901 基本售价： 5135.7 元规格： -

产品信息

概述
货号	AF901
描述	For ELISA the Antibody Pairs information：Capture antibody：AF901；Detection antibody：BAF901; and protein: 901-MP-010
别名	CLGmatrix metalloprotease 1; CLGN; EC 3.4.24; EC 3.4.24.7; Fibroblast collagenase; interstitial collagenase; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloproteinase 1 (interstitial collagenase); Matrix metalloproteinase-1; MMP-1
全称	Matrix Metalloproteinase 1
反应种属	Human
应用	Western Blot,Immunohistochemistry,Knockout Validated,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard
目标/特异性	Detects human MMP-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) MMP‑2, rhMMP-3, rhMMP-7, rhMMP-8, rhMMP-9, rhMMP-10, rhMMP-12, rhMMP-13, and rhMMP-16 is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard :
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4312 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK J. Immunol. Methods, 2012;378(1):72-80. Species: Human Sample Type: Serum Application: ELISA Development Increased expression of matrix metalloproteinase-1 in systemic vessels of preeclamptic women: a critical mediator of vascular dysfunction. Authors: Estrada-Gutierrez G, Cappello RE, Mishra N, Romero R, Strauss JF, Walsh SW Am. J. Pathol., 2011;178(1):451-60. Species: Human Sample Type: Cell Lysates Application: WB TLR4 Protein Contributes to Cigarette Smoke-induced Matrix Metalloproteinase-1 (MMP-1) Expression in Chronic Obstructive Pulmonary Disease. Authors: Geraghty P, Dabo AJ, DArmiento J J. Biol. Chem., 2011;286(34):30211-8. Species: Human Sample Type: Cell Lysates Application: WB Development and validation of sandwich ELISA microarrays with minimal assay interference. Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA J. Proteome Res., 2008;7(6):2406-14. Species: Human Sample Type: Serum Application: ELISA Microarray Development Guggulsterone blocks IL-1beta-mediated inflammatory responses by suppressing NF-kappaB activation in fibroblast-like synoviocytes. Authors: Lee YR, Lee JH, Noh EM, Kim EK, Song MY, Jung WS, Park SJ, Kim JS, Park JW, Kwon KB, Park BH Life Sci., 2008;82(23):1203-9. Species: Human Sample Type: Cell Lysates Application: WB Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes. Authors: Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK FEBS Lett., 2007;581(9):1928-32. Species: Human Sample Type: Cell Culture Supernates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human MMP-1 Phe20-Asn469 Accession # P03956
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, alpha -1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL-1 beta, IGFBP-3, IGFBP-5, pro-MMP-2, and pro-MMP-9. A significant role of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into ¾, ¼ fragments. However, as the list of substrates above illustrates, the role of MMP-1 is more diverse than originally envisaged, and may involve enzyme cascades, cytokine regulation, and cell surface molecule modulation. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes, and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Cawston, T.E. (2004) in Interstitial Collagenase. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 472.

参考图片

Detection of Human MMP‑1 by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line and HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP‑1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MMP‑1 in Human Ovarian Cancer Tissue. MMP‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human MMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MMP‑1 in Human Prostate Tissue. MMP‑1 was detected in immersion fixed paraffin-embedded sections of human prostate tissue using Goat Anti-Human MMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.