| 货号 | AF838 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：AF838；Detection antibody：BAF838; and protein: 838-4B-100 |
| 别名 | 4-1BB ligand receptor; 4-1BB; CD137 antigen; CD137; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9 |
| 反应种属 | Human |
| 应用 | Western Blot,Flow Cytometry,Immunohistochemistry,Agonist Activity,CyTOF-ready,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard |
| 目标/特异性 | Detects 4‑1BB/TNFRSF9/CD137 in direct ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) 4-1BB, recombinant human (rh) NGF R, rhTNF RI and rhTNF RII is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 0.25 µg/106cells Immunohistochemistry: 5-25 µg/mL Agonist Activity: Measured by its ability to co-stimulate IFN-gamma secretion by human T cells in the presence of anti-CD3. The ED50 for this effect is typically 5 - 20 μg/mL. Immunocytochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Targeting peripheral blood pro-inflammatory cytotoxic lymphocytes by inhibiting CD137 expression: novel potential treatment for COPD. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human 4‑1BB/TNFRSF9/CD137 Leu24-Gln186 Accession # Q07011 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Measured by its ability to co-stimulate IFN-gamma secretion by human T cells in the presence of anti-CD3. The ED50 for this effect is typically 5 - 20 μg/mL. |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | 4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The 255 amino acid human 4-1BB is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30 kDa glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% amino acid identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, 4-1BBL, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of 4-1BB by 4-1BBL or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. 4-1BB signals through the TFAF2‑NIK pathway resulting in activation of NF-kappa B and ultimately promoting proliferation and survival of T cells. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of 4‑1BB/TNFRSF9/CD137 in PHA-treated Human T Cells by Flow Cytometry. Human T cells were treated for 48 hours with 5 μg/mL PHA then stained with Goat Anti-Human 4‑1BB/TNFRSF9/ CD137 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF838) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108) and PE-conjugated anti-human CD3 (Catalog # FAB100P). Quad marker was set based on control antibody (Catalog # AB‑108‑C) staining. | |
4‑1BB/TNFRSF9/CD137 in Human Tonsil. 4‑1BB/TNFRSF9/CD137 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human 4‑1BB/TNFRSF9/CD137 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF838) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
