Human IL-17 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human IL-17 Affinity Purified Polyclonal Ab (100 UG)

货号： AF-317-NA 基本售价： 5135.7 元规格： -

产品信息

概述
货号	AF-317-NA
别名	CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
全称	Interleukin 17
反应种属	Human
应用	Western Blot,Immunohistochemistry,Immunoprecipitation,CyTOF-ready,Immunocytochemistry,Intracellular Staining by Flow Cytometry,Neutralization
目标/特异性	Detects human IL-17 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant canine IL-17 is observed, approximately 10% cross-reactivity with recombinant human (rh) IL-17F and with recombinant mouse IL‑17 is observed, and less than 1% cross-reactivity with rhIL-17B, rhIL-17C, rhIL-17D, and rhIL‑17E is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 1-15 µg/mL Immunoprecipitation: 3 µg/100 µg cell lysate Immunocytochemistry: 5-15 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND₅₀) is typically 0.02-0.12 µg/mL in the presence of 15 ng/mL Recombinant Human IL‑17.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy Authors: Neal L Millar Sci Rep, 2016;6(0):27149. Species: Human Sample Type: Whole Tissue Application: IHC - Not specified Atopy and Inhaled Corticosteroid Use Associate with Fewer IL-17+ Cells in Asthmatic Airways PLoS ONE, 2016;11(8):e0161433. Species: Human Sample Type: Whole Tissue Application: IHC - Paraffin embedded Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues Lab Invest, 2016;0(0):. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Association of Lung Inflammatory Cells with Small Airways Function and Exhaled Breath Markers in Smokers - Is There a Specific Role for Mast Cells? Authors: Nussbaumer-Ochsner Y, Stolk J, Ferraz da Silva L, van Schadewijk A, de Jeu R, Prins F, Mauad T, Rabe K, Hiemstra P PLoS ONE, 2015;10(6):e0129426. Species: Human Sample Type: Whole Tissue Application: IHC Not Specified Sera from remitting and secondary progressive multiple sclerosis patients disrupt the blood-brain barrier. Authors: Shimizu F, Tasaki A, Sano Y, Ju M, Nishihara H, Oishi M, Koga M, Kawai M, Kanda T PLoS ONE, 2014;9(3):e92872. Species: Human Sample Type: Serum Application: Neut The effect of adalimumab on key drivers in the pathogenesis of psoriasis. Authors: Hendriks A, van der Velden H, Wolberink E, Seyger M, Schalkwijk J, Zeeuwen P, de Jong E, Pasch M, van Erp P, van de Kerkhof P Br J Dermatol, 2014;170(3):571-80. Species: Human Sample Type: Whole Tissue Application: IHC Upregulation of IL-17A/F from human lung tissue explants with cigarette smoke exposure: implications for COPD. Authors: Chang Y, Al-Alwan L, Alshakfa S, Audusseau S, Mogas A, Chouiali F, Nair P, Baglole C, Hamid Q, Eidelman D Respir Res, 2014;15(1):145. Species: Human Sample Type: tissue Application: IHC Paraffin-embedded Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients. Authors: Liu, Xiaosun, Jin, Hailong, Zhang, Geer, Lin, Xianke, Chen, Chao, Sun, Jianyi, Zhang, Yu, Zhang, Qing, Yu, Jiren PLoS ONE, 2014;9(9):e106834. Species: Human Sample Type: Whole Tissue Application: IHC Not Specified In vivo induction of cutaneous inflammation results in the accumulation of extracellular trap-forming neutrophils expressing RORgammat and IL-17. Authors: Keijsers R, Hendriks A, van Erp P, van Cranenbroek B, van de Kerkhof P, Koenen H, Joosten I J Invest Dermatol, 2014;134(5):1276-84. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded IL-17A induces endothelial inflammation in systemic sclerosis via the ERK signaling pathway. Authors: Xing X, Yang J, Yang X, Wei Y, Zhu L, Gao D, Li M PLoS ONE, 2013;8(12):e85032. Species: Human Sample Type: Whole Cells Application: Neut Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays. Authors: Todd J, Simpson P, Estis J, Torres V, Wub A Cytokine, 2013;64(3):660-5. Species: Human Sample Type: Plasma Application: ELISA Development Interleukin-17A+ cell counts are increased in systemic sclerosis skin and their number is inversely correlated with the extent of skin involvement. Authors: Truchetet M, Brembilla N, Montanari E, Lonati P, Raschi E, Zeni S, Fontao L, Meroni P, Chizzolini C Arthritis Rheum, 2013;65(5):1347-56. Species: Human Sample Type: Whole Tissue Application: IHC - Paraffin embedded Chemotherapy activates cancer-associated fibroblasts to maintain colorectal cancer-initiating cells by IL-17A. Authors: Lotti, Fiorenza, Jarrar, Awad M, Pai, Rish K, Hitomi, Masahiro, Lathia, Justin, Mace, Adam, Gantt, Gerald A, Sukhdeo, Kumar, DeVecchio, Jennifer, Vasanji, Amit, Leahy, Patrick, Hjelmeland, Anita B, Kalady, Matthew, Rich, Jeremy N J Exp Med, 2013;210(13):2851-72. Species: Human Sample Type: whole cells Application: IF Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke. Authors: Gelderblom M, Weymar A, Bernreuther C, Velden J, Arunachalam P, Steinbach K, Orthey E, Arumugam T, Leypoldt F, Simova O, Thom V, Friese M, Prinz I, Holscher C, Glatzel M, Korn T, Gerloff C, Tolosa E, Magnus T Blood, 2012;120(18):3793-802. Species: Human Sample Type: tissue Application: IHC Paraffin-embedded Induction of endothelial nitric oxide synthase expression by IL-17 in human vascular endothelial cells: implications for vascular remodeling in transplant vasculopathy. Authors: Liu AC, Lee M, McManus BM, Choy JC J. Immunol., 2012;188(3):1544-50. Species: Human Sample Type: tissue Application: IHC RASA1 maintains the lymphatic vasculature in a quiescent functional state in mice. Authors: Lapinski PE, Kwon S, Lubeck BA J. Clin. Invest., 2012;122(2):733-47. Species: Human Sample Type: Whole Cells Application: Neut Interleukin-17 expression in neutrophils and Th17 cells in cutaneous T-cell lymphoma associated with neutrophilic infiltrate of the skin. Authors: Fontao L, Brembilla NC, Masouye I, Kaya G, Prins C, Dupin N, Saurat JH, Chizzolini C, Piguet V Br. J. Dermatol., 2012;166(3):687-9. Species: Human Sample Type: Whole Tissue Application: IHC Differential pattern and prognostic significance of CD4+, FOXP3+ and IL-17+ tumor infiltrating lymphocytes in ductal and lobular breast cancers. Authors: Droeser R, Zlobec I, Kilic E, Guth U, Heberer M, Spagnoli G, Oertli D, Tapia C BMC Cancer, 2012;12(0):134. Species: Human Sample Type: Whole Tissue Application: IHC Expression of the T helper 17-associated cytokines IL-17A and IL-17F in asthma and COPD. Authors: Doe C, Bafadhel M, Siddiqui S, Desai D, Mistry V, Rugman P, McCormick M, Woods J, May R, Sleeman MA, Anderson IK, Brightling CE Chest, 2011;138(5):1140-7. Species: Human Sample Type: Whole Tissue Application: IHC IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma. Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S Clin. Cancer Res., 2011;17(24):7529-38. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis. Authors: Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C Br. J. Dermatol., 2011;164(6):1228-34. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Overrepresentation of IL-17A and IL-22 producing CD8 T cells in lesional skin suggests their involvement in the pathogenesis of psoriasis. Authors: Res PC, Piskin G, de Boer OJ, van der Loos CM, Teeling P, Bos JD, Teunissen MB PLoS ONE, 2010;5(11):e14108. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes. Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A J. Immunol., 2010;184(9):4880-8. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded IL-17 induces monocyte migration in rheumatoid arthritis. Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM J. Immunol., 2009;182(6):3884-91. Species: Human Sample Type: Whole Cells Application: Neut IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response. Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A J. Allergy Clin. Immunol., 2009;123(1):59-66.e4. Species: Human Sample Type: Whole Cells Application: Neut Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers. Authors: Nistala K, Moncrieffe H, Newton KR, Varsani H, Hunter P, Wedderburn LR Arthritis Rheum., 2008;58(3):875-87. Species: Human Sample Type: Whole Tissue Application: IHC Increased numbers of circulating polyfunctional Th17 memory cells in patients with seronegative spondylarthritides. Authors: Jandus C, Bioley G, Rivals JP, Dudler J, Speiser D, Romero P Arthritis Rheum., 2008;58(8):2307-17. Species: Human Sample Type: Whole Cells Application: ICC Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum. Authors: Witowski J, Ksiazek K, Warnecke C, Kuzlan M, Korybalska K, Tayama H, Wisniewska-Elnur J, Pawlaczyk K, Trominska J, Breborowicz A, Jorres A Kidney Int., 2007;71(6):514-25. Species: Human Sample Type: Whole Cells Application: Neut Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism. Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D J. Immunol., 2005;174(4):2376-86. Species: Human Sample Type: Whole Cells Application: Neut IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H J. Immunol., 2005;175(9):6177-89. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression. Authors: Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P Am. J. Pathol., 2004;164(2):409-17. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IL-17 Ile20-Ala155 Accession # Q16552
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has recently been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation.
运输条件	Blue Ice
存放说明	-20℃
参考文献	B Cell Chemotaxis, Migration, and Adhesion Cytokines and Receptors in Tfh Cell Differentiation and Function Development and Characterization of Monoclonal Antibody Clone 41802 to Human IL-17A gamma delta T Cells Helper T (Th) Cells IL-17 Family IL-17 is Produced by T-cells Interactive Pathway: IL-17 Family Signaling Pathways LTi Cells Macrophage Activation Markers Mini-review: Interleukin 17 (IL-17) Multiple Sclerosis and Myelin Related Molecules Natural Killer T (NKT) Cells NCR-ILC3 Neuroinflammation T Cell Cytokine Signaling Technical Note: IL-17 Family Antibodies for Flow Cytometry Th17 Cells Th17 Differentiation: An Evolving Target for Multiple Sclerosis Therapy

参考图片

Detection of Human IL‑17 by Western Blot.

Western blot shows lysates of human primary naïve CD4⁺ T cells, along with whole cell lysates (WCL) and conditioned-media supernatant (Supe) of human primary differentiated Th17 cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human
IL‑17 Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF‑317‑NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑17 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑17/IL‑17A in Human Tonsil. IL‑17/IL‑17A was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑17/IL‑17A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunoprecipitation of Human IL‑17.

Human IL-17 was immunoprecipitated from 100 μg of human primary differentiated Th17 cell lysate following incubation with 3 µg Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) or control antibody (Catalog # AB-108-C) overnight at 4 °C. IL-17-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.

IL‑6 Secretion Induced by IL‑17 and Neutralization by Human IL‑17 Antibody.

Recombinant Human IL‑17 (Catalog # 317-ILB) stimulates IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts in a dose-dependent manner (orange line), as measured by the mouse IL‑6 Quantikine ELISA Kit (Catalog # M6000B). IL‑6 secretion elicited by Recombinant Human IL‑17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). The ND₅₀ is typically
0.02‑0.12 µg/mL.

Detection of IL‑17 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL Ca²⁺ ionomycin for 16 hours, then stained with Goat Anti-Human IL‑17 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF‑317‑NA, dark orange filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.