Human IL-1 beta/IL-1F2 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human IL-1 beta/IL-1F2 Affinity Purified Polyclonal Ab (100 UG)

货号： AF-201-NA 基本售价： 5135.7 元规格： -

产品信息

概述
货号	AF-201-NA
别名	catabolin; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
全称	Interleukin 1 beta
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human IL-1 beta /IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30%‑75% cross-reactivity with recombinant mouse IL‑1 beta, recombinant porcine IL-1 beta, recombinant rat IL-1 beta, recombinant canine IL-1 beta, recombinant equine IL-1 beta, recombinant cotton rat IL-1 beta, recombinant feline IL‑1 beta, recombinant rhesus monkey IL-1 beta, and recombinant guinea pig IL-1 beta is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 0.004-0.02 µg/mL in the presence of 50 pg/mL Recombinant Human IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS Authors: Yong Hwan Park Nat Immunol, 2016;0(0):. Species: Human Sample Type: Cell Culture Supernates Application: WB Efficacy and Pharmacology of the NLRP3 Inflammasome Inhibitor CP-456,773 (CRID3) in Murine Models of Dermal and Pulmonary Inflammation J Immunol, 2016;0(0):. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Cutting Edge: Inflammasome Activation in Primary Human Macrophages Is Dependent on Flagellin. Authors: Kortmann J, Brubaker S, Monack D J Immunol, 2015;195(3):815-9. Species: Human Sample Type: Cell Culture Supernates Application: WB Acid-dependent Interleukin-1 (IL-1) Cleavage Limits Available Pro-IL-1beta for Caspase-1 Cleavage. Authors: Edye M, Brough D, Allan S J Biol Chem, 2015;290(42):25374-81. Species: Human Sample Type: recombinant protein Application: WB A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases. Authors: Coll R, Robertson A, Chae J, Higgins S, Munoz-Planillo R, Inserra M, Vetter I, Dungan L, Monks B, Stutz A, Croker D, Butler M, Haneklaus M, Sutton C, Nunez G, Latz E, Kastner D, Mills K, Masters S, Schroder K, Cooper M, ONeill L Nat Med, 2015;21(3):248-55. Species: Mouse Sample Type: Cell Lysates Application: WB Prostaglandin E2 Inhibits NLRP3 Inflammasome Activation through EP4 Receptor and Intracellular Cyclic AMP in Human Macrophages. Authors: Sokolowska M, Chen L, Liu Y, Martinez-Anton A, Qi H, Logun C, Alsaaty S, Park Y, Kastner D, Chae J, Shelhamer J J Immunol, 2015;194(11):5472-87. Species: Human Sample Type: Cell Lysates Application: WB IL-34- and M-CSF-induced macrophages switch memory T cells into Th17 cells via membrane IL-1alpha. Authors: Foucher E, Blanchard S, Preisser L, Descamps P, Ifrah N, Delneste Y, Jeannin P Eur J Immunol, 2015;45(4):1092-102. Species: Human Sample Type: Whole Cells Application: Bioassay Complement C5a potentiates uric acid crystal-induced IL-1beta production. Authors: An L, Mehta P, Xu L, Turman S, Reimer T, Naiman B, Connor J, Sanjuan M, Kolbeck R, Fung M Eur J Immunol, 2014;44(12):3669-79. Species: Human Sample Type: Cell Lysates Application: WB The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP. Authors: Lee G, Subramanian N, Kim A, Aksentijevich I, Goldbach-Mansky R, Sacks D, Germain R, Kastner D, Chae J Nature, 2012;492(7427):123-7. Species: Mouse Sample Type: Cell Culture Supernates Application: WB The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes. Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P J. Immunol., 2011;186(7):4175-82. Species: Human Sample Type: Whole Cells Application: Neut Increased expression of interleukin-1beta and its novel splice variant in canine hearts with volume overload. Authors: Kiczak L, Paslawska U, Bania J, Ugorski M, Sambor I, Kochman A, Blach J, Chelmonska-Soyta A Cytokine, 2008;44(3):352-60. Species: Canine Sample Type: Whole Tissue Application: IHC Paraffin-embedded ADAMTS-1 metalloproteinase promotes tumor development through the induction of a stromal reaction in vivo. Authors: Rocks N, Paulissen G, Quesada-Calvo F, Munaut C, Gonzalez ML, Gueders M, Hacha J, Gilles C, Foidart JM, Noel A, Cataldo DD Cancer Res., 2008;68(22):9541-50. Species: Human Sample Type: Whole Cells Application: Neut Interleukin 1 in oviductal tissues of viviparous, oviparous, and ovuliparous species of amphibians. Authors: Jantra S, Bigliardi E, Brizzi R, Ietta F, Bechi N, Paulesu L Biol. Reprod., 2007;76(6):1009-15. Species: Amphibian - B. bufo (common toad) Sample Type: Tissue Homogenates Application: WB Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo. Authors: Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE Photochem. Photobiol., 2004;80(2):231-5. Species: Human Sample Type: Whole Tissue Application: IHC Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey. Authors: Sengupta J, Dhawan L, Ghosh D Cytokine, 2003;24(6):277-85. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IL-1 beta /IL-1F2 Ala117-Ser269 Accession # NP_000567
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus monkey and 67-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Cancer Biomarkers Chronic IL-1 beta-induced Neuroinflammation Is it Really that Bad? Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis ICC / IHC Images: IL-1 beta/IL-1F2 IL-1 Family Infectious Diseases Inflammasomes: Intracellular Regulators of Pathogen Inflammatory Mediators Interactive Pathway: IL-1 Family Signaling Pathways Isolation, Culture, and Identification of Natural Killer Cells LTi Cells Macrophage Activation Markers Mini-review: Interleukin 1 Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) NCR-ILC3 Neuroinflammation NOD-like Receptors and the Inflammasome Platelets Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th17 Cells Th9 Cells

参考图片

Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑1 beta /IL‑1F2 in Human PBMCs. IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human
IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 beta /IL‑1F2 Antibody. Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA). The ND₅₀ is typically 0.004-0.02 µg/mL in the presence of concanavalin A (1.25 µg/mL).