| 货号 | AB-270-NA |
| 别名 | C-C motif chemokine 3; MIP1-(a); AI323804; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP-1 alpha; Mip1a; MIP-1alpha; MIP1-alpha; Scya3 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human CCL3/MIP-1 alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human MIP-1 beta is observed, less than 15% cross-reactivity with recombinant mouse MIP-1 alpha is observed and less than 1% cross-reactivity with rhMIP-1δ, rmMIP-1 gamma, rmMIP-2, and rhMIP-3 alpha is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically ≤6 µg/mL in the presence of 5 ng/mL Recombinant Human CCL3/MIP‑1 alpha isoform LD78a. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Serum amyloid A chemoattracts immature dendritic cells and indirectly provokes monocyte chemotaxis by induction of cooperating CC and CXC chemokines. | |
| 纯化方式 | Protein A or G purified |
| 免疫原 | E. coli-derived recombinant human CCL3/MIP-1 alpha (R&D Systems, Catalog # 270-LD) Ala27-Ala92 Accession # P10147 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 1 mg/mL in sterile PBS. |
| 背景 | The macrophage inflammatory proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP-1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP-1 alpha. Mature MIP-1 alpha and MIP-1 beta in both human and mouse share approximately 70% homology at the amino acid level. The MIP‑1 proteins are members of the beta (C-C) subfamily of chemokines. Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Human CCL3/MIP‑1 alpha Antibody. Recombinant Human CCL3/MIP‑1 alpha (Catalog # 270-LD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant HumanCCL3/MIP-1 alpha (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL3/MIP-1 alpha Polyclonal Antibody (Catalog # AB-270-NA). The ND50 is typically ≤6 µg/mL. |
