Human CD25/IL-2 R alpha Polyclonal Ab (1 MG)
货号：
AB-223-NA 基本售价：
5135.7 元 规格：
-
产品信息
概述| 货号 | AB-223-NA |
| 别名 | CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR |
全称 | Interleukin 2 Receptor alpha | | 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL)
|
| 目标/特异性 | Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) IL‑2 R beta, rhIL-2 R gamma, and rhIL-15 R is observed. |
| 使用方法 | Western Blot: 1 µg/mL
Neutralization: Measured by its ability to neutralize IL‑2-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑2.
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| 来源 | Polyclonal Goat IgG |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine) |
| 纯化方式 | Protein A or G purified |
| 免疫原 | recombinant human CD25/IL-2 R alpha extracellular domain |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 1 mg/mL in sterile PBS. |
| 背景 | Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Acute Lymphoblastic Leukemia
- Acute Myeloid Leukemia
- B Cell Activation
- B Cell Markers
- Cancer Biomarkers
- Common gamma Chain Receptor Family
- Cytokines and Receptors in Allergy and Asthma
- Cytokines and Receptors in Tfh Cell Differentiation and Function
- Group 1 ILCs
- Group 2 ILCs
- Human CD Marker Products
- ICC / IHC Images: CD25/IL-2 R alpha
- Interactive Pathway: IL-2 Signaling and its Primary Biological Effects in Different Immune Cell Types
- Isolation, Culture, and Identification of Natural Killer Cells
- LTi Cells
- Lymphoma
- NCR+ILC3
- NCR-ILC3
- Neuroinflammation
- Preeclampsia
- Receptors in the Jak/STAT Pathway
- Regulatory T Cells (Tregs)
- T Cell Cytokine Signaling
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参考图片Cell Proliferation Induced by IL‑2 and Neutralization by Human CD25/IL‑2 R alpha Antibody. Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD25/IL‑2 R alpha Polyclonal Antibody (Catalog # AB-223-NA). The ND 50 is typically 5-20 µg/mL. |
