Mouse/Rat Neuropilin-1 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Mouse/Rat Neuropilin-1 Affinity Purified Polyclonal Ab (100 UG)

货号： AF566 基本售价： 5076.2 元规格： -

产品信息

概述
货号	AF566
别名	BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; neuropilin-1; NRP1; NRPNP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165RCD304
反应种属	Mouse/Rat
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human Neuropilin‑1 is observed, and less than 1% cross-reactivity with recombinant human Neuropilin-2, recombinant mouse Neuropilin-2, and recombinant rat Neuropilin-2 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.3-1 µg/mL of this antibody will block 50% of the binding of 20 ng/mL of Recombinant Human VEGF₁₆₅(Catalog #293-VE) to immobilized Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566‑NNS) coated at 2 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding. Also, 1-5 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human Semaphorin 3A Fc Chimera (Catalog # 1250-S3) to immobilized Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-NNS) coated at 2 µg/mL (100 µL/well).
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8829 (Human); 18186 (Mouse); 246331 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. NOVA2-mediated RNA regulation is required for axonal pathfinding during development Elife, 2016;5(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC OCT-embedded VEGF189 binds NRP1 and is sufficient for VEGF/NRP1-dependent neuronal patterning in the developing brain. Authors: Tillo M, Erskine L, Cariboni A, Fantin A, Joyce A, Denti L, Ruhrberg C Development, 2015;142(2):314-9. Species: Mouse Sample Type: Whole Tissue Application: IHC - Not specified Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation. Authors: Plein A, Calmont A, Fantin A, Denti L, Anderson N, Scambler P, Ruhrberg C J Clin Invest, 2015;125(7):2661-76. Species: Mouse Sample Type: Whole Tissue Application: IHC Not Specified Inhibition of neuronal apoptosis and axonal regression ameliorates sympathetic atrophy and hemodynamic alterations in portal hypertensive rats. Authors: Ezkurdia N, Raurell I, Rodriguez S, Gonzalez A, Esteban R, Genesca J, Martell M PLoS ONE, 2014;9(1):e84374. Species: Rat Sample Type: tissue Application: IHC Paraffin-embedded Astrocyte-encoded positional cues maintain sensorimotor circuit integrity. Authors: Molofsky A, Kelley K, Tsai H, Redmond S, Chang S, Madireddy L, Chan J, Baranzini S, Ullian E, Rowitch D Nature, 2014;509(7499):189-94. Species: Rat Sample Type: Whole Cells Application: Neut Fluorochrome-based definition of naturally occurring Foxp3(+) regulatory T cells of intra- and extrathymic origin. Authors: Petzold C, Steinbronn N, Gereke M, Strasser R, Sparwasser T, Bruder D, Geffers R, Schallenberg S, Kretschmer K Eur J Immunol, 2014;44(12):3632-45. Species: Mouse Sample Type: Whole Cells Application: Flow Stability and function of regulatory T cells is maintained by a neuropilin-1-semaphorin-4a axis. Authors: Delgoffe, Greg M, Woo, Seng-Ryo, Turnis, Meghan E, Gravano, David M, Guy, Cliff, Overacre, Abigail, Bettini, Matthew, Vogel, Peter, Finkelstein, David, Bonnevier, Jody, Workman, Creg J, Vignali, Dario A Nature, 2013;501(7466):252-6. Species: Mouse Sample Type: Cell Lysates Application: IP Semaphorin 3A suppresses tumor growth and metastasis in mice melanoma model. Authors: Chakraborty G, Kumar S, Mishra R PLoS ONE, 2012;7(3):e33633. Species: Human Sample Type: Whole Cells Application: Neut Neuropilin 1 is expressed on thymus-derived natural regulatory T cells, but not mucosa-generated induced Foxp3+ T reg cells. J. Exp. Med., 2012;209(10):1723. Species: Mouse Sample Type: Whole Cells Application: Flow Neuropilin-1 is expressed by breast cancer stem-like cells and is linked to NF-kappaB activation and tumor sphere formation. Authors: Glinka Y, Mohammed N, Subramaniam V, Jothy S Biochem. Biophys. Res. Commun., 2012;425(4):775-80. Species: Human Sample Type: Whole Cells Application: Flow Neuropilin 1 deficiency on CD4+Foxp3+ regulatory T cells impairs mouse melanoma growth. J. Exp. Med., 2012;209(11):2001-16. Species: Mouse Sample Type: Whole Cells Application: Flow IL-17-producing invariant NKT cells in lymphoid organs are recent thymic emigrants identified by neuropilin-1 expression. Authors: Milpied P, Massot B, Renand A, Diem S, Herbelin A, Leite-de-Moraes M, Rubio MT, Hermine O Blood, 2011;118(11):2993-3002. Species: Mouse Sample Type: Whole Cells Application: Flow Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development. Authors: Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY J. Clin. Invest., 2010;120(5):1694-707. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Alphav beta3 integrin limits the contribution of neuropilin-1 to vascular endothelial growth factor-induced angiogenesis. Authors: Robinson SD, Reynolds LE, Kostourou V, Reynolds AR, da Silva RG, Tavora B, Baker M, Marshall JF, Hodivala-Dilke KM J. Biol. Chem., 2009;284(49):33966-81. Species: Mouse Sample Type: Tissue Homogenates Application: WB Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice. Authors: Zacchigna S, Pattarini L, Zentilin L, Moimas S, Carrer A, Sinigaglia M, Arsic N, Tafuro S, Sinagra G, Giacca M J. Clin. Invest., 2008;118(6):2062-75. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen BIG-2 mediates olfactory axon convergence to target glomeruli. Authors: Kaneko-Goto T, Yoshihara S, Miyazaki H, Yoshihara Y Neuron, 2008;57(6):834-46. Species: Mouse Sample Type: Tissue Homogenates Application: WB VEGF promotes vascular sympathetic innervation. Authors: Marko SB, Damon DH Am. J. Physiol. Heart Circ. Physiol., 2008;294(6):H2646-52. Species: Rat Sample Type: Cell Lysates Application: WB Semaphorin and neuropilin co-expression in motoneurons sets axon sensitivity to environmental semaphorin sources during motor axon pathfinding. Authors: Moret F, Renaudot C, Bozon M, Castellani V Development, 2007;134(24):4491-501. Species: Chicken Sample Type: Whole Tissue Application: IHC Frozen Modulating Sema3A signal with a L1 mimetic peptide is not sufficient to promote motor recovery and axon regeneration after spinal cord injury. Authors: Mire E, Thomasset N, Jakeman LB, Rougon G Mol. Cell. Neurosci., 2007;37(2):222-35. Species: Mouse Sample Type: Whole Tissue Application: IHC Gating of Sema3E/PlexinD1 signaling by neuropilin-1 switches axonal repulsion to attraction during brain development. Authors: Chauvet S, Cohen S, Yoshida Y, Fekrane L, Livet J, Gayet O, Segu L, Buhot MC, Jessell TM, Henderson CE, Mann F Neuron, 2007;56(5):807-22. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1 Phe22-Ala810 (Lys811Arg), Ser829-Asp854 Accession # Q9QWJ9
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. The amino acid sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Human CD Marker Products ICC / IHC Images: Neuropilin-1 Plasmacytoid Dendritic Cells Regulatory T Cells (Tregs) Semaphorins, Plexin Receptors, and Related Molecules VEGF Family

参考图片

Detection of Neuropilin-1 in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Neuropilin‑1 in Embryonic Rat Spinal Cord.
Neuropilin‑1 was detected in immersion fixed frozen sections of embryonic rat spinal cord (15 d.p.c.) using 5 µg/mL Goat Anti‑Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neuropilin‑1 in Rat Spinal Cord. Neuropilin‑1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the dorsal horn. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.