Human PDGF R alpha Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human PDGF R alpha Affinity Purified Polyclonal Ab (100 UG)

货号： AF-307-NA 基本售价： 5076.2 元规格： -

产品信息

概述
货号	AF-307-NA
别名	alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA
全称	Platelet-derived Growth Factor Receptor alpha
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse PDGF R alpha, recombinant human (rh) PDGF R beta, rhFGF R2, and rhFGF R3 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize PDGF‑AA-induced proliferation in the WS-1 human fetal skin fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 1-6 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑AA.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5156 (Human); 18595 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Identification and characterization of PDGFRalpha+ mesenchymal progenitors in human skeletal muscle. Authors: Uezumi A, Fukada S, Yamamoto N, Ikemoto-Uezumi M, Nakatani M, Morita M, Yamaguchi A, Yamada H, Nishino I, Hamada Y, Tsuchida K Cell Death Dis, 2014;5(0):e1186. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Telocytes in Crohn's disease. Authors: Milia A, Ruffo M, Manetti M, Rosa I, Conte D, Fazi M, Messerini L, Ibba-Manneschi L J Cell Mol Med, 2013;17(12):1525-36. Species: Human Sample Type: Whole Tissue Application: IHC - Paraffin embedded Arhgef15 promotes retinal angiogenesis by mediating VEGF-induced Cdc42 activation and potentiating RhoJ inactivation in endothelial cells. Authors: Kusuhara, Sentaro, Fukushima, Yoko, Fukuhara, Shigetom, Jakt, Lars Mar, Okada, Mitsuhir, Shimizu, Yuri, Hata, Masayuki, Nishida, Kohji, Negi, Akira, Hirashima, Masanori, Mochizuki, Naoki, Nishikawa, Shin-Ich, Uemura, Akiyoshi PLoS ONE, 2012;7(9):e45858. Species: Mouse Sample Type: Whole Tissue Application: IHC Platelet-derived growth factor-alpha receptor activation is required for human cytomegalovirus infection. Authors: Soroceanu L, Akhavan A, Cobbs CS Nature, 2008;455(7211):391-5. Species: Human Sample Type: Cell Lysates Application: IP Distinctions between fetal and adult human platelet-derived growth factor-responsive neural precursors. Authors: Chojnacki A, Kelly JJ, Hader W, Weiss S Ann. Neurol., 2008;64(2):127-42. Species: Human Sample Type: Whole Cells Application: ICC Human bone marrow activates the Akt pathway in metastatic prostate cells through transactivation of the alpha-platelet-derived growth factor receptor. Authors: Dolloff NG, Russell MR, Loizos N, Fatatis A Cancer Res., 2007;67(2):555-62. Species: Human Sample Type: Cell Lysates Application: WB Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells. Authors: Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A Blood, 2006;107(5):1896-902. Species: Human Sample Type: Whole Cells Application: ICC Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas. Authors: Shikada Y, Yonemitsu Y, Koga T, Onimaru M, Nakano T, Okano S, Sata S, Nakagawa K, Yoshino I, Maehara Y, Sueishi K Cancer Res., 2005;65(16):7241-8. Species: Human Sample Type: Cell Lysates Application: Immunodepletion Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty. Authors: Nili N, Cheema AN, Giordano FJ, Barolet AW, Babaei S, Hickey R, Eskandarian MR, Smeets M, Butany J, Pasterkamp G, Strauss BH Am. J. Pathol., 2003;163(3):869-78. Species: Rabbit Sample Type: Cell Lysates Application: WB Content and activity of cAMP response element-binding protein regulate platelet-derived growth factor receptor-alpha content in vascular smooth muscles. Authors: Watson PA, Vinson C, Nesterova A, Reusch JE Endocrinology, 2002;143(8):2922-9. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PDGF R alpha Gln24-Glu524 Accession # P16234
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF‑AA, PDGF‑BB, and PDGF‑AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB and -AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell, and liver endothelial cells. Soluble PDGF R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.

参考图片

Cell Proliferation Induced by PDGF‑AA and Neutralization by Human PDGF R alpha Antibody.

Recombinant Human PDGF‑AA (Catalog # 221-AA) stimulates proliferation in the WS-1 human fetal skin fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF‑AA (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑307‑NA). The ND₅₀ is typically 1-6 µg/mL.

PDGF R alpha in Human Breast Cancer Tissue.

PDGF R alpha was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA) at 15 µg/mL overnight at
4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

PDGF R alpha in Human Ovary.

PDGF R alpha was detected in immersion fixed paraffin-embedded sections of human ovarian array using Goat Anti-Human PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

PDGF R alpha in Human Osteosarcoma. PDGF R alpha was detected in immersion fixed paraffin-embedded sections of human osteosarcoma using Goat Anti-Human PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-307-NA) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.