Human/Rat GDNF Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human/Rat GDNF Affinity Purified Polyclonal Ab (100 UG)

货号： AF-212-NA 基本售价： 5076.2 元规格： -

产品信息

概述
货号	AF-212-NA
别名	Astrocyte-derived trophic factor; ATF; ATF1; ATF2; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; hGDNF; HSCR3
全称	Glial Cell line-derived Growth Factor
反应种属	Human/Rat
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human and rat GDNF in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize GDNF-induced proliferation in the SH‑SY5Y human neuroblastoma cell line. The Neutralization Dose (ND₅₀) is typically 0.25-1 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2668 (Human); 25453 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. beta-Catenin overexpression in the metanephric mesenchyme leads to renal dysplasia genesis via cell-autonomous and non-cell-autonomous mechanisms. Authors: Sarin S, Boivin F, Li A, Lim J, Svajger B, Rosenblum N, Bridgewater D Am J Pathol, 2014;184(5):1395-410. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: implications for stem cell biology and tissue repair. Authors: Wang T, Bruggeman K, Sheean R, Turner B, Nisbet D, Parish C J Biol Chem, 2014;289(21):15044-51. Species: Human Sample Type: recombinant protein Application: ELISA detection Functional neuroprotection and efficient regulation of GDNF using destabilizing domains in a rodent model of Parkinson's disease. Authors: Quintino L, Manfre G, Wettergren E, Namislo A, Isaksson C, Lundberg C Mol Ther, 2013;21(12):2169-80. Species: Human Sample Type: Cell Lysates Application: WB A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys. Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N PLoS ONE, 2012;7(6):e39036. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Tissue Application: IHC Frozen Interventional MRI-guided putaminal delivery of AAV2-GDNF for a planned clinical trial in Parkinson's disease. Authors: Richardson RM, Kells AP, Rosenbluth KH, Salegio EA, Fiandaca MS, Larson PS, Starr PA, Martin AJ, Lonser RR, Federoff HJ, Forsayeth JR, Bankiewicz KS Mol. Ther., 2011;19(6):1048-57. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Tissue Application: IHC Frozen Pharmacokinetics and bioactivity of glial cell line-derived factor (GDNF) and neurturin (NTN) infused into the rat brain. Authors: Hadaczek P, Johnston L, Forsayeth J, Bankiewicz KS Neuropharmacology, 2010;58(7):1114-21. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Response of aged parkinsonian monkeys to in vivo gene transfer of GDNF. Authors: Emborg ME, Moirano J, Raschke J, Bondarenko V, Zufferey R, Peng S, Ebert AD, Joers V, Roitberg B, Holden JE, Koprich J, Lipton J, Kordower JH, Aebischer P Neurobiol. Dis., 2009;36(2):303-11. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Tissue Application: IHC Frozen Efficient gene therapy-based method for the delivery of therapeutics to primate cortex. Authors: Kells AP, Hadaczek P, Yin D, Bringas J, Varenika V, Forsayeth J, Bankiewicz KS Proc. Natl. Acad. Sci. U.S.A., 2009;106(7):2407-11. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Tissue Application: IHC Direct muscle delivery of GDNF with human mesenchymal stem cells improves motor neuron survival and function in a rat model of familial ALS. Authors: Suzuki M, McHugh J, Tork C, Shelley B, Hayes A, Bellantuono I, Aebischer P, Svendsen CN Mol. Ther., 2008;16(12):2002-10. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Identification of neuregulin as a factor required for formation of aligned spermatogonia. Authors: Hamra FK, Chapman KM, Nguyen D, Garbers DL J. Biol. Chem., 2007;282(1):721-30. Species: Rat Sample Type: Whole Cells Application: Neut SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation. Authors: Ai X, Kitazawa T, Do AT, Kusche-Gullberg M, Labosky PA, Emerson CP Development, 2007;134(18):3327-38. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats. Authors: Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O Stroke, 2006;37(9):2361-7. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Human neural stem cell grafts ameliorate motor neuron disease in SOD-1 transgenic rats. Authors: Xu L, Yan J, Chen D, Welsh AM, Hazel T, Johe K, Hatfield G, Koliatsos VE Transplantation, 2006;82(7):865-75. Species: Rat Sample Type: Whole Tissue Application: IHC Glial cell-line-derived neurotropic factor and its receptors are expressed by germinal and somatic cells of the rat testis. Authors: Fouchecourt S, Godet M, Sabido O, Durand P J. Endocrinol., 2006;190(1):59-71. Species: Rat Sample Type: Cell Lysates Application: WB Anatomical basis of glial cell line-derived neurotrophic factor expression in the striatum and related basal ganglia during postnatal development of the rat. Authors: Oo TF, Ries V, Cho J, Kholodilov N, Burke RE J. Comp. Neurol., 2005;484(1):57-67. Species: Rat Sample Type: Whole Cells Application: ICC Therapeutic immunization protects dopaminergic neurons in a mouse model of Parkinsons disease. Authors: Benner EJ, Mosley RL, Destache CJ, Lewis TB, Jackson-Lewis V, Gorantla S, Nemachek C, Green SR, Przedborski S, Gendelman HE Proc. Natl. Acad. Sci. U.S.A., 2004;101(25):9435-40. Species: Mouse Sample Type: Whole Tissue Application: IHC Rapid axoglial signaling mediated by neuregulin and neurotrophic factors. Authors: Esper RM, Loeb JA J. Neurosci., 2004;24(27):6218-27. Species: Chicken Sample Type: Whole Cells Application: Neut Response of motoneurons to neonatal sciatic nerve axotomy in Bax-knockout mice. Authors: Sun W, Oppenheim RW Mol. Cell. Neurosci., 2003;24(4):875-86. Species: Mouse Sample Type: In Vivo Application: In vivo Recombinant adeno-associated viral vector (rAAV) delivery of GDNF provides protection against 6-OHDA lesion in the common marmoset monkey (Callithrix jacchus). Authors: Eslamboli A, Cummings RM, Ridley RM, Baker HF, Muzyczka N, Burger C, Mandel RJ, Kirik D, Annett LE Exp. Neurol., 2003;184(1):536-48. Species: Primate - Callitrix jacchus (common marmoset) Sample Type: Whole Tissue Application: IHC Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron fate. Authors: Honma M, Namikawa K, Mansur K, Iwata T, Mori N, Iizuka H, Kiyama H J. Neurochem., 2002;82(4):961-75. Species: Rat Sample Type: Whole Tissue Application: IHC Immunohistochemical localization of GDNF in the human hippocampal formation from prenatal life to adulthood. Authors: Pina Serra M, Quartu M, Ambu R, Follesa P, Del Fiacco M Brain Res., 2002;928(1):138-46. Species: Human Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived and Mouse myeloma cell line NS0-derived recombinant human GDNF Arg109-Ile211 Accession # P39905
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.
运输条件	Blue Ice
存放说明	-20℃
参考文献	ALS Related Molecules GDNF Family GDNF Family Ligands GDNF: A Novel Treatment for Parkinson’s Disease? ICC / IHC Images: GDNF Mini-review: Glial Cell Line-derived Neurotrophic Factor (GDNF) Neurotrophic Factors and Receptors Parkinsons Disease Related Molecules

参考图片

GDNF and GFR alpha ‑1 in Rat Dorsal Root Ganglia. GDNF and GFR alpha ‑1 were detected in perfusion fixed frozen sections of rat dorsal root ganglia using 10 µg/mL Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF-212-NA) and 15 µg/mL Biotinylated Goat Anti-Rat GFR alpha ‑1 Antigen Affinity-purified Polyclonal Antibody (red; Catalog # BAF560). Tissue was incubated with primary antibodies overnight at 4 °C. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Proliferation Induced by GDNF and Neutralization by Goat GDNF Antibody. Recombinant Human GDNF (Catalog # 212-GD) induces proliferation in the SH‑SY5Y human neuroblastoma cell line in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR) in a dose-dependent manner (orange line), as measured by Rezazurin (Catalog # AR002). Under these conditions, proliferation elicited by GDNF is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-212-NA). The ND₅₀ is typically 0.25-1 μg/mL.