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货号： bs-1284R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1284R
- 英文名称
- Bak
- 中文名称
- Bcl-2同源拮抗剂抗体
- 别 名
- Apoptosis Regulator Bak; BAK 1; BAK; BAK like; Bak NT; BAK1; Bcl 2 homologous antagonist/killer; Bcl 2 like 7 protein; Bcl2 homologous antagonist killer; Bcl2 like 7 Protein; BCL2-antagonist/killer 1; BCL2L7; CDN 1; CDN1; Cell death inhibitor 1; MGC117255; MGC3887; NBak; Pro apoptotic protein BAK; BAX_MOUSE.
- Specific References (2) | bs-1284R has been referenced in 2 publications.[IF=3.73] Zhu, Zhongling, et al. "Regulation of Cell Transformation by Rb-Controlled Redox Homeostasis." PLoS ONE 9.7 (2014): e102582. WB ; Mouse.PubMed:25019272[IF=5.29] Huang, Chuen-Lin, et al. "Paraquat Induces Cell Death Through Impairing Mitochondrial Membrane Permeability." Molecular Neurobiology (2015): 1-20. Rat.PubMed:25947082
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 免疫学 细胞凋亡
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 23kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from mouse Bak:21-120/209
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008]
Function:
In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.
Subunit:
Belongs to the Bcl-2 family.
Subcellular Location:
Mitochondrion membrane.
Tissue Specificity:
Expressed in a wide variety of tissues.
Similarity:
Belongs to the Bcl-2 family.
SWISS:
O08734
Gene ID:
12018
Database links:Entrez Gene: 578 Human
Entrez Gene: 12018 Mouse
Omim: 600516 Human
SwissProt: Q16611 Human
SwissProt: Q6I9T6 Human
SwissProt: O08734 Mouse
Unigene: 485139 Human
Unigene: 2443 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Bak属于bcl-2基因家族中的成员，具有抑制凋亡的作用。BAK基因是新发现的细胞凋亡促进因子，BAK含BH1和BH2结构域，其功能类似于BAX；拮抗BCL2、BCL-XL的抗凋亡效应。
- 产品图片
Sample:Lung (Mouse) Lysate at 30 ug
Primary: Anti-Bak(bs-1284R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 5000 dilution;
Predicted band size : 23kD
Observed band size : 23kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Bak Polyclonal Antibody, Unconjugated(bs-1284R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Bak Polyclonal Antibody, Unconjugated(bs-1284R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela(blue).
Primary Antibody:Rabbit Anti- Bak antibody(bs-1638R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Antibody (bs-1284R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1284R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.