Human Cathepsin S Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human Cathepsin S Affinity Purified Polyclonal Ab (100 UG)

货号： AF1183 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF1183
描述	For ELISA the Antibody Pairs information：Capture antibody：AF1183；Detection antibody：BAF1183; and protein: 1183-CY-010
别名	cathepsin S; CTSS; EC 3.4.22; EC 3.4.22.27; FLJ50259; MGC3886
反应种属	Human
应用	Western Blot,Immunohistochemistry,Immunoprecipitation,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human Cathepsin S in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human Cathepsin A, B, C, D, E, L, V, and X/Z/P is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize activation and the resulting activity of Recombinant Human Cathepsin S (0.25 µg/mL, Catalog # 1183-CY) in cleaving the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (10 µM, Catalog # ES002). The Neutralization Dose (ND₅₀) is typically 2 µg/mL.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1520 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors Sci Rep, 2016;6(0):29256. Species: Human Sample Type: Cell Lysates Application: WB Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer. Authors: Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K PLoS ONE, 2015;10(4):e0120348. Species: Mouse Sample Type: Whole Tissue Application: IHC-OCT embedded Cathepsin S cannibalism of cathepsin K as a mechanism to reduce type I collagen degradation. Authors: Barry, Zachary, Platt, Manu O J Biol Chem, 2012;287(33):27723-30. Species: Human Sample Type: protein Application: WB Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer. Authors: Shree T, Olson OC, Elie BT Genes Dev., 2011;25(23):2465-79. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Antibody targeting of Cathepsin S induces antibody-dependent cellular cytotoxicity. Authors: Kwok HF, Buick RJ, Kuehn D Mol. Cancer, 2011;10(0):147. Species: Human Sample Type: Whole Cells Application: Flow Human cysteine cathepsins are not reliable markers of infection by Pseudomonas aeruginosa in cystic fibrosis. Authors: Naudin C, Joulin-Giet A, Couetdic G, Plesiat P, Szymanska A, Gorna E, Gauthier F, Kasprzykowski F, Lecaille F, Lalmanach G PLoS ONE, 2011;6(9):e25577. Species: Human Sample Type: Sputum Application: WB A multiplex immunoassay for human adipokine profiling. Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E Clin. Chem., 2010;56(0):1320. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model. Authors: Wendt W, Schulten R, Stichel CC, Lubbert H J. Neurochem., 2009;110(6):1931-41. Species: Mouse Sample Type: Cell Lysates Application: WB Upregulation of cathepsin S in the aging and pathological nervous system of mice. Authors: Wendt W, Lubbert H, Stichel CC Brain Res., 2008;1232(0):7-20. Species: Mouse Sample Type: Tissue Homogenates Application: WB Distinct roles for cysteine cathepsin genes in multistage tumorigenesis. Authors: Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, Hanahan D, Joyce JA Genes Dev., 2006;20(5):543-56. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Cathepsin S Gln17-Ile331 Accession # P25774
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II-associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1-16), a pro region (residues 17-114), and the mature enzyme (residues 115-331) (3-5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages, and other phagocytic cells.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104 - 1107, Academic Press, San Diego. Turk, V. et al. (2001) EMBO J. 20:4629. Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258. Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530. Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.

参考图片

Cathepsin S in Human Lymph Node. Cathepsin S was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human Cathepsin S Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1183) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.