| 货号 | AB-269-NA |
| 别名 | CD121 antigen-like family member A; CD121a antigen; CD121a; IL1R; IL1R1; IL1RT1; IL-1RT1; IL-1RT-1; Interleukin 1 Receptor 1; interleukin 1 receptor, type I; interleukin receptor 1; interleukin-1 receptor type 1; p80 | 全称 | Interleukin 1 Receptor I |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human IL-1 RI in direct ELISAs and Western blots. In direct ELISAs and Western blots,approximately 5‑10% cross-reactivity with recombinant human IL‑1 RII is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced inhibition of proliferation in the A375s2 human melanoma cell line. The Neutralization Dose (ND50) is typically 5-10 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL‑1 beta /IL‑1F2. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3554 (Human); 16177 (Mouse) |
| 纯化方式 | Protein A or G purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-1 RI |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 1 mg/mL in sterile PBS. |
| 背景 | Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex. An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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IL‑1 beta /IL‑1F2 Inhibition of Cell Proliferation and Neutralization by Human IL‑1 RI Antibody. Recombinant Human IL‑1 beta / IL‑1F2 (Catalog # 201-LB) inhibits proliferation in the A375s2 human melanoma cell line in a dose-dependent manner (orange line). Activity elicited by Recombinant Human IL‑1 beta /IL‑1F2 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑1 RI Polyclonal Antibody (Catalog # AB‑269‑NA). The ND50 is typically 5-10 µg/mL. |
