Human PD-ECGF/Thymidine Phosphorylase Polyclonal Ab (1 MG)【科瑞奥博】

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Human PD-ECGF/Thymidine Phosphorylase Polyclonal Ab (1 MG)

货号： AB-229-NA 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AB-229-NA
别名	dThdPase; EC 2.4.2; EC 2.4.2.4; ECGF; ECGF1; ECGF1TP; endothelial cell growth factor 1 (platelet-derived); Gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; PD-ECGF; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine Phosphorylase; TPase; TYMP
全称	Platelet-derived Endothelial Cell Growth Factor
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human PD-ECGF/Thymidine Phosphorylase in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with ____________ is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize PD‑ECGF-induced proliferation in HUVEC human umbilical vein endothelial cells. Usuki, K. et al. (1990) Cell Regulation 1:577. The Neutralization Dose (ND₅₀) is typically 10-25 µg/mL in the presence of 150 ng/mL Recombinant Human PD‑ECGF.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1890 (Human); 72962 (Mouse)
纯化方式	Protein A or G purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PD-ECGF/Thymidine Phosphorylase Ala11-Gln482 Accession # P19971.2
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 1 mg/mL in sterile PBS.
背景	PD-ECGF was originally isolated as a platelet-derived endothelial cell mitogen (based on its ability to stimulate 3H-thymidine incorporation) and its cDNA was cloned from a human placental cDNA library. In solution, PD-ECGF exists as a non-disulfide linked homodimer. The sequence of human PD-ECGF is identical to that of human thymidine phosphorylase (dThdPase).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Haraguchi, M. et al. (1994) Nature 368:198. Toi, M. et al. (2005) Lancet Oncol. 6:158. Akiyama, S. et al. (2004) Canc. Sci. 95:851.

参考图片

Cell Proliferation Induced by PD‑ECGF/Thymidine Phosphorylase and Neutralization by Human PD‑ECGF/Thymidine Phosphorylase Antibody.

Recombinant Human PD‑ECGF/Thymidine Phosphorylase (Catalog # 229‑PE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PD‑ECGF/Thymidine Phosphorylase (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PD‑ECGF/Thymidine Phosphorylase Polyclonal Antibody (Catalog # AB-229-NA). The ND₅₀ is typically 10‑25 µg/mL.