| 货号 | AB-218-NA |
| 别名 | Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL-11Oprelvekin; interleukin 11; interleukin-11 | 全称 | Interleukin 11 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human IL‑11 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 25% cross-reactivity with recombinant mouse IL-11 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize IL‑11-induced proliferation in the T11 mouse plasmacytoma cell line. Nordan, R. P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND50) is typically 10-20 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑11. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3589 (Human); 16156 (Mouse); 171040 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells. | |
| 纯化方式 | Protein A or G purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑11 Pro22-Leu199 Accession # P20809 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 1 mg/mL in sterile PBS. |
| 背景 | Interleukin 11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1 alpha -stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive murine plasmacytoma cell line T1165. IL-11 was also independently discovered as an adipogenesis inhibitory factor (AGIF). The human IL-11 cDNA encodes a 199 amino acid residue precursor polypeptide with a 21 amino acid residue hydrophobic signal that is processed proteolytically to generate the 178 amino acid residue mature protein. IL-11 contains no cysteine residues or potential glycosylation sites. IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cell. IL-11, in the presence of IL-3 or SCF, has also been shown to stimulate erythropoiesis. Among nonhematopoietic cell populations, IL-11, like IL-6 and LIF, can stimulate the synthesis of hepatic acute-phase proteins. Consistent with the in vitro functions of IL‑11, in vivo administration of rhIL-11 in normal mice was found to enhance the generation of Ig producing cells and platelets, and to increase the cycling rates of bone marrow-derived CFU-GM, BFU-E, and CFU-GEMM progenitors. IL-11 exerts its biological activities through binding to a specific high-affinity receptor having an apparent molecular mass of 150 kDa. Although the IL-11 binding subunit of the receptor complex has not yet been cloned, evidence suggests that, similar to IL-6, leukemia inhibitory factor, oncostatin M, and ciliary neurotrophic factor, IL-11 utilizes the IL-6 signal transducer, gp130, for signal transduction. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Cell Proliferation Induced by IL‑11 and Neutralization by Human IL‑11 Antibody. Recombinant Human IL‑11 (Catalog # 218‑IL) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑11 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑11 Polyclonal Antibody (Catalog # AB‑218‑NA). The ND50 is typically 10‑20 µg/mL. |
