Human IL-10 Polyclonal Ab (1 MG)【科瑞奥博】

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Human IL-10 Polyclonal Ab (1 MG)

货号： AB-217-NA 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AB-217-NA
别名	CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
全称	Interleukin 10
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human IL‑10 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant equine IL‑10, recombinant canine IL‑10, recombinant porcine IL‑10, and recombinant feline IL‑10 is observed, and less than 10% cross-reactivity with recombinant mouse IL‑10, recombinant rat IL‑10, and recombinant cotton rat IL‑10 is observed.
使用方法	Western Blot: 1 µg/mL Simple Western: 10 µg/mL Neutralization: Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line [Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507]. The Neutralization Dose (ND₅₀) is typically 3-15 µg/mL in the presence of 5 ng/mL Recombinant Human IL‑10.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade. Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D J Immunol, 2012;189(12):5590-601. Species: Human Sample Type: whole cells Application: Bioassay Toll-like receptor 9 signaling by CpG-B oligodeoxynucleotides induces an apoptotic pathway in human chronic lymphocytic leukemia B cells. Authors: Liang X, Moseman EA, Farrar MA Blood, 2010;115(24):5041-52. Species: Human Sample Type: Whole Cells Application: Neut Does granulocyte colony-stimulating factor ameliorate the proinflammatory response in human meningococcal septic shock? Authors: Rojahn A, Brusletto B, Ovstebo R, Haug KB, Kierulf P, Brandtzaeg P Crit. Care Med., 2008;36(9):2583-9. Species: Human Sample Type: Plasma Application: Immunodepletion
纯化方式	Protein A or G purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10 Ser19-Asn178 Accession # P22301
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 1 mg/mL in sterile PBS.
背景	Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL-22, IL-24, and IL-26/AK155 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2 - 5). Mature human IL-10 shares 72%‑86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (6, 8, 9). The IL‑10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (10). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (11‑13). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (14‑16). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172. Hsu, D.-H. et al. (1990) Science 250:830. Windsor, W.T. et al. (1993) Biochemistry 32:8807. Syto, R. et al. (1998) Biochemistry 37:16943. Kotenko, S.V. et al. (1997) EMBO J. 16:5894. Kotenko, S.V.et al. (2000) J. Biol. Chem. 276:2725. Hor, S. et al. (2004) J. Biol. Chem. 279:33343. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269. Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.

参考图片

Detection of Human IL‑10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL‑10 Polyclonal Antibody (Catalog # AB-217-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cell Proliferation Induced by IL‑10 and Neutralization by Human IL‑10 Antibody. Recombinant Human IL‑10 (Catalog # 217-IL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑10 Polyclonal Antibody (Catalog # AB-217-NA). The ND₅₀ is typically 3-15 µg/mL.