Human CXCL8/IL-8 Polyclonal Ab (1 MG)【科瑞奥博】

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Human CXCL8/IL-8 Polyclonal Ab (1 MG)

货号： AB-208-NA 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AB-208-NA
别名	3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1
全称	Interleukin 8
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human CXCL8/IL-8 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) GRO alpha, rhGRO beta, and rhGRO gamma is observed, and less than 1% cross-reactivity with recombinant feline CXCL8/IL-8, recombinant porcine CXCL8/IL-8, and recombinant canine CXCL8/IL-8 is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CXCL8/IL‑8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 5-20 µg/mL in the presence of 20 ng/mL Recombinant Human CXCL8/IL‑8.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Evidence for chemokine synergy during neutrophil migration in ARDS Thorax, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Neut Expression and regulation of chemokines in murine and human type 1 diabetes. Authors: Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D Diabetes, 2012;61(2):436-46. Species: Human Sample Type: Whole Cells Application: Ctrl Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth. Authors: Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E Nat. Med., 2011;17(11):1498-503. Species: Human Sample Type: Whole Cells Application: Neut Tumor-derived chemokine MCP-1/CCL2 is sufficient for mediating tumor tropism of adoptively transferred T cells. Authors: Brown CE, Vishwanath RP, Aguilar B, Starr R, Najbauer J, Aboody KS, Jensen MC J. Immunol., 2007;179(5):3332-41. Species: Human Sample Type: Whole Cells Application: Neut The effect of respiratory synctial virus on chemokine release by differentiated airway epithelium. Authors: Mellow TE, Murphy PC, Carson JL, Noah TL, Zhang L, Pickles RJ Exp. Lung Res., 2004;30(1):43-57. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified
免疫原	E. coli-derived recombinant human CXCL8/IL‑8 Ser28-Ser99 Accession # P10145
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 1 mg/mL in sterile PBS.
背景	CXCL8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc. CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. CXCL8 was also reported to be angiogenic both in vivoandin vitro.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Adipocytokines Cancer Biomarkers Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis CXC Chemokines and Receptors Diabetes HIF Transcription Factors ICC / IHC Images: CXCL8/IL-8 Infectious Diseases Inflammatory Mediators Macrophage Activation Markers Megakaryocytes Neuroinflammation Platelets

参考图片

Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Human CXCL8/IL‑8 Antibody.

Recombinant Human CXCL8/IL‑8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL8/IL‑8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL8/IL‑8 Polyclonal Antibody (Catalog # AB-208-NA). The ND₅₀ is typically 5-20 µg/mL.