Rat CXCL3/CINC-2 alpha/beta Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Rat CXCL3/CINC-2 alpha/beta Affinity Purified Polyclonal Ab (100 UG)

货号： AF517 基本售价： 4580.5 元规格： -

产品信息

概述
货号	AF517
别名	chemokine (C-X-C motif) ligand 3; CINC-2; CINC-2b; C-X-C motif chemokine 3; Dcip1; Gm1960; GRO gamma; GRO3 oncogene; GRO3; GROG; GRO-gamma; GRO-gamma(1-73); Growth-regulated protein gamma; Macrophage inflammatory protein 2-beta; melanoma growth stimulatory activity gamma; MGSA gamma; MIP2B; MIP-2b; MIP2-beta; SCYB3
反应种属	Rat
应用	Western Blot,Neutralization
目标/特异性	Detects rat CXCL3/CINC-2 alpha / beta in direct ELISAs and Western blots. In direct ELISAs, almost 100% cross-reactivity with recombinant rat (rr) CINC-2 alpha is observed, approximately 50% cross-reactivity with recombinant mouse (rm) MIP-2 is observed, 35% cross‑reactivity with rmKC is observed, 15% cross-reactivity with rrCINC-1 is observed, and 10% cross-reactivity with recombinant human (rh) IL‑8, rhGRO alpha, rhGRO beta, and rhGRO gamma is observed.
使用方法	Western Blot Neutralization: Measured by its ability to neutralize CXCL3/GRO gamma /CINC‑2/DCIP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.4-2.0 µg/mL in the presence of 0.05 µg/mL Recombinant Rat CXCL3/GRO gamma /CINC‑2/DCIP‑1.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2921 (Human); 330122 (Mouse); 171551 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant rat CXCL3/CINC-2 alpha / beta Arg33-Leu100 Accession # NP_612531
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CXCL3/GRO gamma, also known as DCIP-1 in mouse and CINC-2 in rat, is a member of the CXC chemokine family. Human CXCL3/GRO gamma is 107 amino acids (aa) in length, which includes a proposed signal peptide (aa 1–34), with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 65% and 59% aa sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl1 gene is homologous to human CXCL3/GRO gamma protein. CXCL3/GRO gamma is chemotactic for granulocytes, including neturophils.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Chemokines and Receptors in Angiogenesis CXC Chemokines and Receptors Inflammatory Mediators Macrophage Activation Markers

参考图片

Chemotaxis Induced by CXCL3/CINC‑2 beta and Neutralization by Rat CXCL3/
CINC‑2 alpha / beta Antibody.

Recombinant Rat CXCL3/
CINC‑2 beta (Catalog # 517-CB) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL3/
CINC‑2 beta (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CXCL3/
CINC-2 alpha / beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF517). The ND₅₀ is typically 0.4-2.0 µg/mL.