Human IFN-alpha Polyclonal Ab (100000 UN)
货号：
31101-1 基本售价：
3668.3 元 规格：
-
产品信息
概述| 货号 | 31101-1 |
| 别名 | IFNalpha; IFN-alpha |
全称 | Interferon alpha | | 反应种属 | Human |
| 应用 | Neutralization |
| 目标/特异性 | Human IFN-alpha |
| 使用方法 | Neutralization: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-alpha ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) J. Virol. 37, 755-758] using A549 cells and EMCV was used as the challenge virus [Budd et al. (1985) Canc. Chem. Pharm. 12:39]. In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined by use of a laboratory standard calibrated with respect to the international reference standard for Hu-IFN-alpha provided by the National Institutes of Health [Gxa01-901-535]. This material is prepared specifically for effective neutralization of Hu-IFN-alpha.
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| 来源 | Polyclonal Rabbit Serum |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 3440 (Human) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TLR4 ligands induce IFN-alpha production by mouse conventional dendritic cells and human monocytes after IFN-beta priming.
Authors: Richez C, Yasuda K, Watkins AA
J. Immunol., 2009;182(2):820-8.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: ELISA Development |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human IFN-alpha |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 背景 | The most common use of this product has been in a pool with Rabbit Anti-Human Interferon Beta (PBL # 31410) and Mouse Anti-IFNAR2 (PBL# 21385) to block the activity of all Type I IFNs. Krug et al [Eur. J. Immunol. (2001) 31:2154] used a pool of PBL #31101, #31410 and #21385 to demonstrate PBMC-derived Type I IFN is responsible for increased IFN-gamma secretion after stimulation with CpG ODN. Ito et al. [J. Exp. Med. (2002) 195:1507] used a similar pool to determine that TLR7 stimulated pDC but not mDC induce Th1 development through Type I IFN. Lande et al. [J. Immunol. (2003) 170:1174] used a pool of PBL#31410 and #31101 to show a blockade of autocrine Alpha/Beta action leads to decreased CXCL-10 secretion from Mycobacterium-infected dendritic cells. Jego et al. [Immunity (2003) 19:225] demonstrated the inhibition of IFN-alpha signaling in a co-culture of pDCs with CD40 stimulated B-cells reduces IgG secretion, while inhibition of IFN-beta signaling does not occur. Ku et al. [J. Exp. Med. (2004) 200:917] showed epithelial cells surrounding Varicella Zoster lesions express IFN-alpha by immunohistochemistry (IHC) in a SCID-human skin zenograft model. |
| 运输条件 | Dry Ice |
| 存放说明 | -20℃ |
| 参考文献 | - B Cell Chemotaxis, Migration, and Adhesion
- Cytokines and Receptors in Angiogenesis
- IL-10/Interferon Family
- Infectious Diseases
- Plasmacytoid Dendritic Cells
- Th9 Cells
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