Human IL-23 p19 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human IL-23 p19 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1716-SP 基本售价： 2488.5 元规格： -

产品信息

概述
货号	AF1716-SP
别名	IL-23; IL23A; IL-23A; IL-23-A; IL-23p19; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha
全称	Interleukin 23
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human IL‑23 p19 in direct ELISA and Western blot. In direct ELISA, approximately 50% cross-reactivity with recombinant canine IL-23p19, less then 25% cross-reactivity with recombinant feline IL-23p19, less than 5% cross-reactivity with recombinant mouse IL-23p19, recombinant human IL-12p40, and recombinant rat IL-23p40 is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize IL‑23-induced IL‑17 secretion in mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The Neutralization Dose (ND₅₀) is typically 0.2-0.8 µg/mL in the presence of 0.75 ng/mL Recombinant Human IL‑23 and 10 ng/mL Recombinant Mouse IL‑2.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	51561 (Human); 83430 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F Nature, 2012;484(7395):514-8. Species: Human Sample Type: Whole Cells Application: Neut Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade. Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D J Immunol, 2012;189(12):5590-601. Species: Human Sample Type: whole cells Application: Neut Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis. Authors: Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C Br. J. Dermatol., 2011;164(6):1228-34. Species: Human Sample Type: Whole Tissue Application: IHC Frozen The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes. Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P J. Immunol., 2011;186(7):4175-82. Species: Human Sample Type: Whole Cells Application: Neut TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23. Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M Eur. J. Immunol., 2009;39(5):1221-30. Species: Human Sample Type: Whole Cells Application: Neut IL-23 and IL-12 responses in activated human T cells retrovirally transduced with IL-23 receptor variants. Authors: de Paus RA, van de Wetering D, Van Dissel JT Mol. Immunol., 2008;45(15):3889-95. Species: Human Sample Type: Whole Cells Application: Neut Blockade of TLR9 agonist-induced type I interferons promotes inflammatory cytokine IFN-gamma and IL-17 secretion by activated human PBMC. Authors: Meyers JA, Mangini AJ, Nagai T, Roff CF, Sehy D, van Seventer JM Cytokine, 2006;35(5):235-46. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IL‑23 p19 Arg20-Pro189 Accession # AAG37232
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Human p19 cDNA encodes a 189 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 170 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8^- and CD8⁺ subtypes, however only IL-23 can act directly on CD8⁺ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.
运输条件	Blue Ice
存放说明	4℃
参考文献	Conventional/Classical Dendritic Cells IL-12 Family IL-23: A New Composite Cytokine Inflammatory/Monocyte-derived Dendritic Cells LTi Cells Macrophage Activation Markers Multiple Sclerosis and Myelin Related Molecules NCR+ILC3 NCR-ILC3 Neuroinflammation T Cell Cytokine Signaling Th17 Cells The IL-12 Family of Cytokines & Mechanisms of Intestinal Inflammation

参考图片

IL‑17 Secretion Induced by IL‑23 and Neutralization by Human IL‑23 Antibody. In the presence of Recombinant Mouse IL‑2 (10 ng/mL, Catalog # 402‑ML), Recombinant Human IL‑23 (Catalog # 1290-IL) stimulates IL‑17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL‑17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL‑17 secretion elicited by Recombinant Human IL‑23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1716). The ND₅₀ is typically 0.2-0.8 µg/mL.

Detection of human IL‑23 p19 by Western Blot. Western blot shows lysates of a CHO cell line transfected with human IL-23. A PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL‑23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1716) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑23 p19 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.