| 货号 | AF793-SP |
| 别名 | CXCL7/Thymus Chemokine-1; Thymus Chemokine1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects CXCL7/Thymus Chemokine‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human GRO beta is observed and 5% cross-reactivity with recombinant rat CINC-3 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize CXCL7/Thymus Chemokine‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.8-4 µg/mL in the presence of 40 ng/mL Recombinant Mouse CXCL7/Thymus Chemokine‑1. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 57349 (Mouse); 246358 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CXCR4+CD45- cells are niche forming for osteoclastogenesis via the SDF-1, CXCL7, and CX3CL1 signaling pathways in bone marrow | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse CXCL7/Thymus Chemokine-1 Lys40-Tyr113 Accession # AAG36786 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Mouse CXCL7 cDNA encodes a 113 amino acid residue precursor protein. Signal P prediction program analysis indicates that this precursor protein contains an amino terminal 39 amino acid residue signal peptide and a 74 amino acid mature chemokine. Mouse CXCL7 shares 72% amino acid sequence identity with rat CXCL7. The recombinant mouse CXCL7 produced by R&D Systems has been shown to chemoattract mouse BaF3 cells transfected with human CXCR2, but not human CXCR1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by CXCL7/Thymus Chemokine‑1 and Neutralization by Mouse CXCL7/Thymus Chemokine‑1 Antibody. Recombinant Mouse CXCL7/Thymus Chemokine‑1 (Catalog # 1091-CK) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL7/Thymus Chemokine‑1 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL7/Thymus Chemokine‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF793). The ND50 is typically 0.8-4 µg/mL. | |
CXCL7/Thymus Chemokine‑1 in Mouse Thymus. CXCL7/Thymus Chemokine‑1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CXCL7/Thymus Chemokine‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF793) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
