| 货号 | AF2625-SP |
| 别名 | DKFZp586J1923; EC 3.4.21; EC 3.4.21.-; kallikrein 13; Kallikrein-like protein 4; kallikrein-related peptidase 13; KLK13; KLK-L4kallikrein-13; KLKL4kallikrein-like gene 4 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Kallikrein 13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) Kallikrein 8 and less than 5% cross-reactivity with rhKallikrein 11, rhKallikrein 14, and rhKallikrein 15 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human Kallikrein 13 (1 µg/mL, Catalog # 2625-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (100 µM, Catalog # ES011). The Neutralization Dose (ND50) is typically 8.6 µg/mL. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 26085 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Kallikrein 13 Val19-Ile262 Accession # Q9UKR3 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human tissue Kallikrein 13 (hK13) is a member of the human tissue kallikrein family. The gene encoding for rhK13 is termed KLK13 and it resides on chromosome 19q13.3-4 along with fourteen other members of the family. KLK13 spans about 8.7 kb genomic DNA and the longest transcript is 831 bp, encoding for a protein of 277 amino acids (1). Another five shorter splice variants have also been identified. They are specifically expressed in the testicular tissue and encode for five truncated forms of hK13 (2). Due to the aspartic acid residue in the substrate binding pocket, the enzymatic activity of hK13 is predicted be trypsin-like. It has been shown that recombinant hK13 produced in yeast can cleave synthetic peptides after the arginine residue and some extracellular matrix components (3). However, its exact physiological substrates and functions remain obscure. Despite the lack of knowledge on the physiological function of hK13, several studies have demonstrated that hK13 is implicated with cancer of the breast and ovary and it can serve as a favorable prognostic biomarker for these malignancies (4, 5). The purified, secreted rhK13 is mainly the pro form. When activated by lysyl-endopeptidase, it displays enzymatic activity towards a fluorogenic synthetic peptide. This activity can be inhibited by rhSerpin A5, E1, and F2 (R&D Systems, Catalog # 1266-PI, 1786-PI, and 1470-PI). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Kallikrein 13 in Human Prostate Cancer Tissue. Kallikrein 13 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human Kallikrein 13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2625) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
