| 货号 | AF193-SP |
| 别名 | B cell maturation antigen; B-cell maturation protein; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF17; tumor necrosis factor receptor superfamily, member 17 | 全称 | B Cell Maturation Factor |
| 反应种属 | Human |
| 应用 | Western Blot,Flow Cytometry,Blockade of Receptor-ligand Interaction,CyTOF-ready,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard |
| 目标/特异性 | Detects human BCMA in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhTACI, and recombinant mouse BCMA is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 0.25 µg/106cells Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.3-1.2 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of Recombinant Human APRIL/TNFSF13 (Catalog # 884-AP) to immobilized Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC) coated at 1 µg/mL (100 µL/well). At 15 μg/mL, this antibody will block >90% of the binding. ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 608 (Human); 21935 (Mouse); 287034 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human BCMA/TNFRSF17 Met1-Ala54 Accession # Q6PE46 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human, Primate |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | BCMA, B cell maturation, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of BCMA/TNFRSF17 in U266 Human Cell Line by Flow Cytometry. U266 human myeloma cell line was stained with Goat Anti-Human/Primate BCMA/ TNFRSF17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF193, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). |
