| 货号 | AF1199-SP |
| 别名 | CD266 antigen; CD266; FGF-inducible 14; Fibroblast growth factor-inducible immediate-early response protein 14; Fn14; FN14tumor necrosis factor receptor superfamily member 12A; TNFRSF12; TNFRSF12A; tumor necrosis factor receptor superfamily, member 12A; TWEAKR; tweak-receptor; type I transmembrane protein Fn14 | 全称 | TNF-like and Weak Inducer of Apoptosis Receptor |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human TWEAK R/TNFRSF12 in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse TWEAK R/TNFRSF12 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize TWEAK R/TNFRSF12-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 0.3-1.2 µg/mL in the presence of 30 ng/mL Recombinant Human TWEAK R/TNFRSF12 Fc Chimera and 20 ng/mL Recombinant Human TWEAK/TNFSF12. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 51330 (Human); 27279 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TWEAK/Fn14 system and crescent formation in IgA nephropathy. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | recombinant human TWEAK R/TNFRSF12 Glu28-Trp79 Accession # Q9NP84 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The gene for TNF-related weak inducer of apoptosis receptor (TWEAK R) was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Human TWEAK R cDNA encodes a 129 amino acid (aa) residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain (1‑3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain conatins one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death, cell proliferation, and angiogenesis (2‑6). In new born mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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TWEAK R/TNFRSF12 Inhibition of TWEAK/TNFSF12-dependent Cell Proliferation and Neutralization by Human TWEAK R/TNFRSF12 Antibody. Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (Catalog # 1199‑TW) inhibits Recombinant Human TWEAK/TNFSF12 (Catalog # 1090‑TW) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human TWEAK/TNFSF12 (20 ng/mL) activity elicited by Recombinant Human TWEAK R/TNFRSF12 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TWEAK R/TNFRSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1199). The ND50is typically 0.3-1.2 µg/mL. |
