Mouse Cardiotrophin-1 (CT-1) Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Cardiotrophin-1 (CT-1) Affinity Purified Polyclonal Ab (25 UG)

货号： AF438-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF438-SP
别名	cardiophin 1; cardiotrophin 1; cardiotrophin-1; CT-1CT1; CTF1
反应种属	Mouse
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects Cardiotrophin‑1/CT‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human CT-1 is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize Cardiotrophin‑1/CT‑1-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.08-0.48 µg/mL in the presence of 10 ng/mL Recombinant Mouse Cardiotrophin‑1/CT‑1.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1489 (Human); 13019 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cardiotrophin-1 defends the liver against ischemia-reperfusion injury and mediates the protective effect of ischemic preconditioning. Authors: Iniguez M, Berasain C, Martinez-Anso E, Bustos M, Fortes P, Pennica D, Avila MA, Prieto J J. Exp. Med., 2006;203(13):2809-15. Species: Mouse Sample Type: In Vivo Application: In vivo In vivo electrotransfer of the cardiotrophin-1 gene into skeletal muscle slows down progression of motor neuron degeneration in pmn mice. Authors: Lesbordes JC, Bordet T, Haase G, Castelnau-Ptakhine L, Rouhani S, Gilgenkrantz H, Kahn A Hum. Mol. Genet., 2002;11(14):1615-25. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse Cardiotrophin-1/CT‑1 Ser2-Ala203 Accession # Q60753
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Mouse CT-1 encodes a 203 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third alpha receptor subunit has also been implicated in the receptor complex.
运输条件	Blue Ice
存放说明	4℃
参考文献	Pennica, D. et al. (1996) Cytokine and Growth Factor Reviews 7:81. Robledo, O. et al. (1997) J. Biol. Chem. 272:4855.

参考图片

Cell Proliferation Induced by Cardiotrophin‑1/CT‑1 and Neutralization by Mouse Cardiotrophin‑1/CT‑1 Antibody. Recombinant Mouse Cardiotrophin‑1/CT‑1 (Catalog # 438-CT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Cardiotrophin‑1/CT‑1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Cardiotrophin‑1/CT‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF438). The ND₅₀ is typically 0.08‑0.48 µg/mL.