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Rat ICAM-1/CD54 Affinity Purified Polyclonal Ab (25 UG)

货号: AF583-SP 基本售价: 1378.1 元 规格: -

产品信息

概述
货号AF583-SP
别名BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58
全称Intercellular Adhesion Molecule 1
反应种属Rat
应用Western Blot(0.2 µg/mL)
Immunohistochemistry(5-15 µg/mL)
目标/特异性Detects rat ICAM-1/CD54 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) ICAM-1 and 5% cross-reactivity with recombinant human (rh) ICAM-1, rhICAM‑3, and rmICAM‑2 is observed.
使用方法Western Blot: 0.2 µg/mL
Immunohistochemistry: 5-15 µg/mL
Adhesion Blockade: The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Rat ICAM-1 Fc Chimera (Catalog # 583-IC, 12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.
来源Polyclonal Goat IgG
产品组分
性能
供应商R&D Systems
Entrez Gene IDs3383 (Human); 15894 (Mouse); 25464 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Astaxanthin Inhibits Expression of Retinal Oxidative Stress and Inflammatory Mediators in Streptozotocin-Induced Diabetic Rats.
Authors: Yeh P, Huang H, Yang C, Yang W, Yang C
PLoS ONE, 2016;11(1):e0146438.
Species: Rat
Sample Type: Cell Lysates
Application: WB
Hypothermic preservation of lung allograft inhibits cytokine-induced chemoattractant-1, endothelial leucocyte adhesion molecule, vascular cell adhesion molecule-1 and intracellular adhesion molecule-1 expression.
Authors: Hanusch C, Nowak K, Gill IS, Torlitz P, Rafat N, Mueller AM, Van Ackern KC, Yard B, Beck GC
Clin. Exp. Immunol., 2007;149(2):364-71.
Species: Rat
Sample Type: Whole Tissue
Application: IHC
Aspirin at low-intermediate concentrations protects retinal vessels in experimental diabetic retinopathy through non-platelet-mediated effects.
Authors: Sun W, Gerhardinger C, Dagher Z, Hoehn T, Lorenzi M
Diabetes, 2005;54(12):3418-26.
Species: Rat
Sample Type: Whole Tissue
Application: IHC

纯化方式Antigen Affinity-purified
免疫原Mouse myeloma cell line NS0-derived recombinant rat ICAM-1/CD54
Gln28-Thr493
Accession # Q00238
内毒素水平<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性Rat
标记Unconjugated
溶解方法Reconstitute at 0.2 mg/mL in sterile PBS.
背景

Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and lipolysaccharide (LPS). Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

运输条件Blue Ice
存放说明4℃
参考文献
  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
参考图片
Detection of Rat ICAM‑1/CD54 by Western Blot. Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF583) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ICAM‑1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ICAM‑1/CD54 in Rat Brain. ICAM‑1/CD54 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Rat ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF583) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.