Rat GFR alpha-1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Rat GFR alpha-1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF560-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF560-SP
别名	DKFZp313E1012; DKFZp686J0156; GDNF family receptor alpha 1; GDNF family receptor alpha-1; GDNF R alpha-1; GDNF receptor alpha-1; GDNFRAGFR-ALPHA-1; GDNFR-alpha-1; GDNFRMGC23045; GFRA1; GFR-alpha-1; Glial cell line-derived neurotrophic factor receptor alpha; GPI-linked anchor protein; PI-linked cell-surface accessory protein; RET ligand 1; RET1L; RETL1FLJ10561; TGF-beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; TRNR1FLJ31546
全称	Glial Cell line-derived Neurotrophic Factor Receptor alpha 1
反应种属	Rat
应用	Western Blot(0.2 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects rat GFR alpha ‑1/GDNF R alpha ‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human GFR alpha ‑1 is observed and less than 1% cross-reactivity with recombinant mouse GFR alpha ‑2 is observed.
使用方法	Western Blot: 0.2 µg/mL Immunohistochemistry: 5-15 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 4-10 µg/mL of this antibody will block 50% of the binding of 4 ng/mL of Recombinant Human GDNF (Catalog # 212-GD) to immobilized Recombinant Rat GFR alpha -1 Fc Chimera (Catalog # 560-GR) coated at 0.5 µg/mL (100 µL/well).
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2674 (Human); 25454 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors. Authors: Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L Proc Natl Acad Sci U S A, 2015;112(15):E1946-55. Species: Mouse Sample Type: Whole Tissue Application: IHC - Not specified LIN28A marks the spermatogonial progenitor population and regulates its cyclic expansion. Authors: Chakraborty P, Buaas F, Sharma M, Snyder E, de Rooij D, Braun R Stem Cells, 2014;32(4):860-73. Species: Mouse Sample Type: Whole Tissue Application: IF Retinoic acid availability drives the asynchronous initiation of spermatogonial differentiation in the mouse. Authors: Snyder EM, Small C, Griswold MD Biol. Reprod., 2010;83(5):783-90. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3. Authors: Suzuki H, Sada A, Yoshida S, Saga Y Dev. Biol., 2009;336(2):222-31. Species: Mouse Sample Type: Whole Cells Application: ICC Astrocyte and muscle-derived secreted factors differentially regulate motoneuron survival. Authors: Taylor AR, Gifondorwa DJ, Newbern JM, Robinson MB, Strupe JL, Prevette D, Oppenheim RW, Milligan CE J. Neurosci., 2007;27(3):634-44. Species: Chicken Sample Type: Whole Cells Application: Neut Involvement of GDNF and its receptors in the maturation of the periodontal Ruffini endings. Authors: Igarashi Y, Aita M, Suzuki A, Nandasena T, Kawano Y, Nozawa-Inoue K, Maeda T Neurosci. Lett., 2007;412(3):222-6. Species: Rat Sample Type: Whole Tissue Application: IHC Glial cell line-derived neurotrophic factor: an intraovarian factor that enhances oocyte developmental competence in vitro. Authors: Linher K, Wu D, Li J Endocrinology, 2007;148(9):4292-301. Species: Porcine Sample Type: Cell Lysates Application: WB Nerve growth factor, glial cell line-derived neurotrophic factor and neurturin prevent semaphorin 3A-mediated growth cone collapse in adult sensory neurons. Authors: Wanigasekara Y, Keast JR Neuroscience, 2006;142(2):369-79. Species: Rat Sample Type: Whole Cells Application: ICC Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo. Authors: Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM J. Neurosci., 2006;26(33):8588-99. Species: Mouse Sample Type: Whole Cells Application: ICC Neurotrophin and GDNF family ligands promote survival and alter excitotoxic vulnerability of neurons derived from murine embryonic stem cells. Authors: Lee CS, Tee LY, Dusenbery S, Takata T, Golden JP, Pierchala BA, Gottlieb DI, Johnson EM, Choi DW, Snider BJ Exp. Neurol., 2005;191(1):65-76. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant rat GFR alpha ‑1/GDNF R alpha ‑1 Asp25-Leu445 Accession # Q62997
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and ‑2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an N‑terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.
运输条件	Blue Ice
存放说明	4℃
参考文献	Thompson, J. et al. (1998) Mol. Cell Neurosci.11:117. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.

参考图片

Detection of Rat GFR alpha ‑1/GDNF R alpha ‑1 by Western Blot. Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat GFR alpha ‑1/GDNF R alpha ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GFR alpha ‑1/GDNF R alpha ‑1 at approximately 52 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

GFR alpha ‑1/GDNF R alpha ‑1 in Rat Spinal Cord. GFR alpha ‑1/GDNF R alpha ‑1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GFR alpha ‑1/GDNF R alpha ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spinal cord dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.