| 货号 | AF543-SP |
| 别名 | LIX |
| 反应种属 | Rat |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects rat CXCL5/LIX in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse GCP-2 is observed and less than 5% cross‑reactivity with recombinant human GCP-2 is observed and no cross reactivity with _____________ is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CXCL5/LIX-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 0.2 µg/mL Recombinant Rat CXCL5/LIX. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 20311 (Mouse); 60665 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant rat LIX Ala38-Gln130 Accession # P97885.1 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | LPS-induced CXC chemokine (LIX) is a member of the murine chemokine family. It is 132 amino acids (aa) in length with a predicted molecular weight of 14 kDa prior to cleavage of the N-terminal signal peptide (aa 1–40). LIX has no true human ortholog but is closely related to human CXCL5/ENA-78 and CXCL6/GCP-2. It is widely expressed, and is secreted by multiple cell types including fibroblasts, thymic epithelium, platelets, vascular endothelium, and hepatocytes. LIX acts as a proinflammatory chemokine and is chemotactic for neutrophils. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Chemotaxis Induced by CXCL5/LIX and Neutralization by Rat CXCL5/LIX Antibody. Recombinant Rat CXCL5/LIX (Catalog # 543-RL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL5/LIX (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CXCL5/LIX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF543). The ND50 is typically 0.3-1.5 µg/mL. |
