Rat IL-6 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Rat IL-6 Affinity Purified Polyclonal Ab (25 UG)

货号： AF506-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF506-SP
别名	B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta 2; IFN-beta-2; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
全称	Interleukin 6
反应种属	Rat
应用	Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects rat and mouse IL-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant cotton rat IL‑6 is observed, and less than 1% cross-reactivity with recombinant human IL-6 is observed.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND₅₀) is typically 0.1-0.5 µg/mL in the presence of 0.6 ng/mL Recombinant Rat IL‑6.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone. Authors: Shigemoto-Mogami Y, Hoshikawa K, Goldman J, Sekino Y, Sato K J Neurosci, 2014;34(6):2231-43. Species: Rat Sample Type: Whole Cells Application: Neut Sublytic C5b-9 induces IL-6 and TGF-beta1 production by glomerular mesangial cells in rat Thy-1 nephritis through p300-mediated C/EBPbeta acetylation. Authors: Zhang J, Li Y, Shan K, Wang L, Qiu W, Lu Y, Zhao D, Zhu G, He F, Wang Y FASEB J, 2014;28(3):1511-25. Species: Rats Sample Type: Whole Cells Application: Bioassay Electrical stimulation induces IL-6 in skeletal muscle through extracellular ATP by activating Ca(2+) signals and an IL-6 autocrine loop. Authors: Bustamante M, Fernandez-Verdejo R, Jaimovich E, Buvinic S Am J Physiol Endocrinol Metab, 2014;306(8):E869-82. Species: Rat Sample Type: Whole Cells Application: Neut Bradykinin and nerve growth factor play pivotal roles in muscular mechanical hyperalgesia after exercise (delayed-onset muscle soreness). Authors: Murase S, Terazawa E, Queme F, Ota H, Matsuda T, Hirate K, Kozaki Y, Katanosaka K, Taguchi T, Urai H, Mizumura K J. Neurosci., 2010;30(10):3752-61. Species: Rat Sample Type: In Vivo Application: In vivo Astrocyte-derived interleukin-6 promotes specific neuronal differentiation of neural progenitor cells from adult hippocampus. Authors: Oh J, McCloskey MA, Blong CC J. Neurosci. Res., 2010;88(13):2798-809. Species: Rat Sample Type: Whole Cells Application: Neut Trauma and hemorrhage-induced acute hepatic insulin resistance: dominant role of tumor necrosis factor-alpha. Authors: Xu J, Kim HT, Ma Y, Zhao L, Zhai L, Kokorina N, Wang P, Messina JL Endocrinology, 2008;149(5):2369-82. Species: Rat Sample Type: In Vivo Application: In vivo JAK/STAT3 pathway is activated in spinal cord microglia after peripheral nerve injury and contributes to neuropathic pain development in rat. Authors: Dominguez E, Rivat C, Pommier B, Mauborgne A, Pohl M J. Neurochem., 2008;107(1):50-60. Species: Rat Sample Type: In Vivo Application: In vivo Microglia-derived interleukin-6 and leukaemia inhibitory factor promote astrocytic differentiation of neural stem/progenitor cells. Authors: Nakanishi M, Niidome T, Matsuda S, Akaike A, Kihara T, Sugimoto H Eur. J. Neurosci., 2007;25(3):649-58. Species: Rat Sample Type: Cell Culture Supernates Application: Immunodepletion Mechanism of IL-6-mediated cardiac dysfunction following trauma-hemorrhage. Authors: Yang S, Hu S, Hsieh YC, Choudhry MA, Rue LW, Bland KI, Chaudry IH J. Mol. Cell. Cardiol., 2006;40(4):570-9. Species: Rat Sample Type: Application: Neut Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation. Authors: Barkho BZ, Song H, Aimone JB Stem Cells Dev., 2006;15(3):407-21. Species: Rat Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant rat IL-6 Phe25-Thr211 Accession # P20607
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature rat IL-6 is 187 amino acids (aa) in length and shares 41% and 85% aa sequence identity with human and mouse IL-6, respectively (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).
运输条件	Blue Ice
存放说明	4℃
参考文献	Van Snick, J. (1990) Annu. Rev. Immunol. 8:253. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502. Jones, S.A. (2005) J. Immunol. 175:3468. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227. Northemann, W. et al. (1989) J. Biol. Chem. 264:16072. Murakami, M. et al. (1993) Science 260:1808. Muller-Newen, G. (2003) Sci. STKE 2003:PE40. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.

参考图片

Detection of Rat IL‑6 by Western Blot. Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat
IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑6 in Mouse T Cells. IL‑6 was detected in immersion fixed activated mouse T Cells using 15 µg/mL Rat IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cell Proliferation Induced by IL‑6 and Neutralization by Rat IL‑6 Antibody. Recombinant Rat IL‑6 (Catalog # 506-RL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat
IL‑6 (0.6 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506). The ND₅₀ is typically 0.1-0.5 µg/mL.