| 货号 | AF1190-SP |
| 别名 | AGS2; hN2; neurogenic locus notch homolog protein 2; Notch (Drosophila) homolog 2; notch 2Notch homolog 2 (Drosophila); Notch homolog 2 |
| 反应种属 | Rat |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects rat Notch-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant rat Notch‑1 is observed, and less than 1% cross-reactivity with recombinant mouse Notch‑3 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunohistochemistry: 5-15 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 2-5 µg/mL of this antibody will block 50% of the binding of 200 ng/mL of Recombinant Rat Jagged-1 Fc Chimera (Catalog # 599-JG) to immobilized Recombinant Rat Notch-2 Fc Chimera (Catalog # 1190‑NT) coated at 5 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4853 (Human); 29492 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant rat Notch-2 Leu26-Glu492 Accession # Q9QW30 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Rat Notch-2 is a 300 kDa, type I transmembrane glycoprotein involved in a number of early-event developmental processes (1). In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types. The molecule is synthesized as a 2472 amino acid (aa) precursor that contains a putative 27 aa signal sequence, a 1650 aa extracellular region, a 23 aa transmembrane (TM) segment and a 772 aa cytoplasmic domain (2). The large Notch extracellular domain has 36 EGF-like repeats followed by three notch/Lin-12 repeats (LNR). Of the 36 EGF-like repeats, the 11th and 12thEGF-like repeats have been shown to be both necessary and sufficient for binding the ligands Serrate and Delta, in Drosophila (3). Cell surface Notch receptor is thought to be a heterodimer consisting of the ligand binding extracellular region associated with the remaining transmembrane protein, as a result of post-translational proteolytic cleavage by a furin-like enzyme. Upon ligand binding, additional proteolysis events result in the release of the Notch intracellular domain (NICD). NICD translocates into the nucleus and initiates transcription of Notch-responsive genes (4). Thus Notch acts as both a ligand-binding receptor and a nuclear factor that regulates transcription. In addition, an alternative Notch signaling pathway that is mediated by the full-length, uncleaved form of Notch-1 at the cell surface has been reported to suppress differentiation of myoblasts in response to ligand binding (5). Rat Notch-2 shows 92% and 95% aa identity to human and mouse Notch-2 extracellular domains, respectively. Relative to the extracellular region of rat Notch-1, rat Notch-2 exhibits 56% aa identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Notch‑2 in Rat Brain. Notch‑2 was detected in perfusion fixed frozen sections of rat brain (choroid plexus) using 5 µg/mL Goat Anti-Rat Notch‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1190) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
Notch‑2 in Embryonic Rat Liver. Notch‑2 was detected in immersion fixed frozen sections of embryonic rat liver (15 d.p.c.) using 1.7 µg/mL Goat Anti-Rat Notch‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1190) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
