| 货号 | AF912-SP |
| 别名 | CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35 | 全称 | Interleukin 12 |
| 反应种属 | Porcine |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects porcine IL-12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-12 is observed, and less than 5% cross-reactivity with recombinant mouse IL-12 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IL‑12-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) [Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894]. The Neutralization Dose (ND50) is typically 0.01-0.04 µg/mL in the presence of 0.75 ng/mL Recombinant Porcine IL‑12. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3592 (Human); 16159 (Mouse); 84405 (Rat); 403977 (Canine); 493741 (Feline) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant porcine IL‑12 p35/p40 heterodimer Ile23-Asn324 (p40), Arg26-Ser222 (p35) Accession # Q28938 (p40), Q29053 (p35) |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Porcine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. Some of these IL-12 activities include the induction of IFN-gamma and TNF in resting and activated T and NK cells, the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen, and the enhancement of NK cell proliferation. Current evidence indicates that IL-12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent. Porcine IL-12 subunits p35 and p40 share about 85% homology to the human subunits, but differ by containing a 3 amino acid addition in the p35 subunit and a 4 amino acid deletion in the p40 subunit. Porcine IL-12 induces proliferation of human lymphoblasts and IFN-gamma secretion by human and porcine lymph nodes. Porcine IL-12 has been detected in lymphoid tissues including inguinal and mesenteric lymph nodes, Peyers patches, spleen, and thymus. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by IL‑12 and Neutralization by Porcine IL‑12 Antibody. Recombinant Porcine IL‑12 (Catalog # 912-PL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑12 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF912). The ND50 is typically 0.01-0.04 µg/mL. |
