Porcine GM-CSF Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Porcine GM-CSF Affinity Purified Polyclonal Ab (25 UG)

货号： AF711-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF711-SP
别名	colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; molgramostin; sargramostim
全称	Granulocyte Macrophage Growth Factor
反应种属	Porcine
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects porcine GM‑CSF in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant rat GM‑CSF is observed, 15% cross-reactivity with recombinant human GM‑CSF is observed, and 5% cross-reactivity with recombinant mouse GM‑CSF is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize GM‑CSF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.2-1.0 µg/mL in the presence of 10 ng/mL Recombinant Porcine GM‑CSF.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant porcine GM-CSF Ala18-Lys144 Accession # Q29118
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Porcine
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.
运输条件	Blue Ice
存放说明	4℃
参考文献	Common beta Chain Receptor Family Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Dendritic Cell Development Gametes and Fertilization gamma delta T Cells ICC / IHC Images: GM-CSF Inflammatory Mediators Isolation, Culture, and Identification of Natural Killer Cells Macrophage Activation Markers Myeloid-derived Suppressor Cells (MDSC) Natural Killer T (NKT) Cells T Cell Cytokine Signaling

参考图片

Cell Proliferation Induced by GM‑CSF and Neutralization by Porcine GM‑CSF Antibody. Recombinant Porcine GM‑CSF (Catalog # 711-PG) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine GM‑CSF (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine GM‑CSF Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF711). The ND₅₀ is typically 0.2-1.0 µg/mL.