Porcine TNF-alpha Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Porcine TNF-alpha Affinity Purified Polyclonal Ab (25 UG)

货号： AF690-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF690-SP
别名	APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
全称	Tumor Necrosis Factor alpha
反应种属	Porcine
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects porcine TNF‑ alpha in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the PK‑15 porcine kidney epithelial cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221; Bertoni et al. (1993) J. Immunol. Meth. 160:267. The Neutralization Dose (ND₅₀) is typically 0.2-0.8 µg/mL in the presence of 0.05 ng/mL Recombinant Porcine TNF‑ alpha and 1 µg/mL actinomycin D.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Atorvastatin treatment improves survival and effects of implanted mesenchymal stem cells in post-infarct swine hearts. Authors: Yang YJ, Qian HY, Huang J, Geng YJ, Gao RL, Dou KF, Yang GS, Li JJ, Shen R, He ZX, Lu MJ, Zhao SH Eur. Heart J., 2008;29(12):1578-90. Species: Porcine Sample Type: Tissue Homogenates Application: WB Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expression. Authors: Hadjadj S, Cloutier I, Geoffroy P, Tanguay JF Atherosclerosis, 2007;192(1):25-32. Species: Porcine Sample Type: Whole Tissue Application: IHC Paraffin-embedded Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate. Authors: Zhang F, Hopwood P, Abrams CC, Downing A, Murray F, Talbot R, Archibald A, Lowden S, Dixon LK J. Virol., 2006;80(21):10514-21. Species: Porcine Sample Type: Cell Lysates Application: WB Liposomal delivery of heat shock protein 72 into renal tubular cells blocks nuclear factor-kappaB activation, tumor necrosis factor-alpha production, and subsequent ischemia-induced apoptosis. Authors: Meldrum KK, Burnett AL, Meng X, Misseri R, Shaw MB, Gearhart JP, Meldrum DR Circ. Res., 2003;92(3):293-9. Species: Porcine Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant porcine TNF-alpha Arg78-Leu232 Accession # P23563
内毒素水平	<0.01 EU per 1 μg of the antibody by the LAL method.
生物活性	Porcine
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Porcine TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 176 aa extracellular domain (ECD) (3). Within the ECD, porcine TNF-alpha shares 69%‑86% aa sequence identity with bovine, canine, cotton rat, equine, feline, human, mouse, rat, and rhesus TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11).
运输条件	Blue Ice
存放说明	4℃
参考文献	Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1. Pauli, U. et al. (1989) Gene 81:185. Tang, P. et al. (1996) Biochemistry 35:8216. Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353. Black, R.A. et al. (1997) Nature 385:729. Moss, M.L. et al. (1997) Nature 385:733. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324. Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335. Romanatto, T. et al. (2007) Peptides 28:1050. Hector, J. et al. (2007) Horm. Metab. Res. 39:250.

参考图片

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Porcine TNF‑ alpha Antibody. Recombinant Porcine TNF‑ alpha (Catalog #
690‑PT) induces cytotoxicity in the the PK‑15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Porcine TNF‑ alpha (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF690). The ND₅₀ is typically 0.2-0.8 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

TNF‑ alpha in Porcine PBMCs. TNF‑ alpha was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Goat Anti-Porcine TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF690) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.