Feline IL-2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Feline IL-2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF1890-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF1890-SP
别名	aldesleukin; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
全称	Interleukin 2
反应种属	Feline
应用	Western Blot,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects feline IL‑2 in ELISAs and Western blots. In sandwich immunoassays, approximately 3% cross-reactivity with recombinant canine IL-2 is observed, approximately 1% cross reactivity with recombinant human IL-2 and recombinant porcine IL-2 is observed, and less than 0.2% cross‑reactivity with recombinant mouse IL-2, recombinant equine IL-2, recombinant bovine IL-2, recombinant rat IL-2, and recombinant cotton rat IL-2 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A. J. H. and C. B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND₅₀) is typically 0.03-0.15 µg/mL in the presence of 0.2 ng/mL Recombinant Feline IL‑2 (Cys146Ser).
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Vaccination with vif-deleted feline immunodeficiency virus provirus, GM-CSF, and TNF-alpha plasmids preserves global CD4 T lymphocyte function after challenge with FIV. Authors: Maksaereekul S, Dubie RA, Shen X, Kieu H, Dean GA, Sparger EE Vaccine, 2009;27(28):3754-65. Species: Feline Sample Type: Cell Culture Supernates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant feline IL‑2 Ala21-Thr154 (Cys146Ser) Accession # Q07885
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Feline
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin-2 (IL-2) is a secreted, single chain alpha -helical polypeptide that has potent stimulatory activity for antigen-activated T cells. The feline IL-2 gene encodes a 154 amino acid (aa) precursor protein with a 20 aa signal peptide plus a 134 aa mature segment. There are suggestions that the mature protein may be O‑glycosylated. At the aa sequence level, mature feline IL-2 is 78%, 82%, 60%, 64%, 62%, 75%, 62%, and 76% identical to mature human, canine, mouse, rat, cotton rat, porcine, goat, and equine IL-2, respectively. Mammalian cells known to express IL-2 include CD4⁺ and CD8⁺ T cells, visceral smooth muscle cells, eosinophils, gamma δ T cells, B cells and dendritic cells. The biological activity of IL-2 is mediated by IL-2 receptor complexes consisting of three distinct subunits ( alpha, beta, gamma ) in two combinations. The high-affinity signaling IL-2 receptor complex is a heterotrimer of the IL-2 receptor alpha, beta, gamma subunits. The intermediate signaling complex is a heterodimer of the IL-2 R beta and gamma subunits. The non-ligand binding gamma subunit, referred to as the common gamma subunit ( gamma _c), is also a subunit of the receptor complexes of IL-4, IL-7, IL-9 and IL-15. Functionally, IL-2 is best known for its autocrine and paracrine activity on T cells. On naïve CD8⁺ T cells, high IL-2 levels can induce cell proliferation with a bias towards cytotoxicity. In the presence of low levels of IL-2, CD8⁺ T cells preferentially undergo apoptosis with a bias towards cytokine secretion. IL-2 also seems to play a central role in the expansion and maintenance of CD4⁺CD25⁺ regulatory T cells. This indicates IL-2 may be a key cytokine in the natural suppression of autoimmunity (1-9).
运输条件	Blue Ice
存放说明	4℃
参考文献	Cozzi, P.J. et al. (1993) Biochem. Biophys. Res. Commun. 194:1038. Smith, K.A. (1992) Curr. Opin. Immunol. 4:271. Conradt, H.S. et al. (1989) J. Biol. Chem. 264:17368. Matesanz, F. et al. (1993) Immunogenetics 38:300. Ellery, J.M. and P.J. Nicholls (2002) Cytokine Growth Factor Rev. 13:27. Liparoto, S.F. et al. (2002) Biochemistry 41:2543. Lin, J-X. and W.J. Leonard (1997) Cytokine Growth Factor Rev. 8:313. Sad, S. and L. Krishnan (1999) J. Immunol. 163:2443. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.

参考图片

Cell Proliferation Induced by IL‑2 and Neutralization by Feline IL‑2 Antibody. Recombinant Feline IL‑2 (Cys146Ser) (Catalog #
1890-FL) induces cell proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Feline IL‑2 (Cys146Ser) (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890). The ND₅₀ is typically
0.03-0.15 µg/mL.

IL‑2 in Feline PBMCs. IL‑2 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) stimulated with PMA and calcium ionomycin using Goat Anti-Feline IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.