| 货号 | AF127-SP |
| 别名 | NTN2L; NTN3 |
| 反应种属 | Chicken |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects chicken Netrin‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant chicken Netrin-1 is observed and less than 1% cross-reactivity with recombinant human Netrin-4, recombinant mouse (rm) Netrin-G1a and rmNetrin‑G2a is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 1-5 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Chicken Netrin-2 (Catalog # 127-N2) to immobilized Recombinant Rat UNC5H2 Fc Chimera (Catalog # 1006-UN) coated at 5 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4917 (Human); 396387 (Chicken) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant chicken Netrin-2 Ala16-Pro581 Accession # Q90923 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Chicken |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Netrins are laminin-like axon guidance molecules that are conserved among C. elegans,Drosophila, and vertebrates. The chicken Netrin-2 cDNA encodes a precursor protein that has structural similarity to the N-terminus of B2 or the gamma -chain of laminin. The mature Netrin-2 protein has 581 amino acid (aa) which contains a 245 aa domain VI, a 169 aa domain V with three cysteine/glycine rich EGF repeats and a unique 152 aa domain C that enriches in basic aa residues and contains an RGD motif. Chicken Netrin-2 shares 72% aa identity with chicken Netrin-1, 51% aa identity with C. elegans UNC-6 protein and 57% aa identity with human NTN2L (Netrin‑2‑like). Although only two chicken netrins are known, the number of known mammalian netrins is growing. Mouse Netrin-3 and Netrin-4 were recently reported. Netrin-2 was expressed in the chick brain and spinal cord from E6 through to the adult. In addition, expression of Netrin-2 was observed at various stages in several mesodermally and endodermally derived tissues, including lung, gut, ovary, testes and spleen. Netrins act to both attract and repel the growing axons of a broad range of neuronal cell types during development. These actions are mediated by specific receptor complexes containing either the DCC (colorectal carcinoma) or neogenin, in the case of attractant, or UNC-5-related proteins, in the case of repellent. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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