Mouse Amphiregulin Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse Amphiregulin Affinity Purified Polyclonal Ab (25 UG)

货号： AF989-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF989-SP
别名	amphiregulin; AR; AREG; AREGB; Colorectum cell-derived growth factor; CRDGF; MGC13647; schwannoma-derived growth factor; SDGF
反应种属	Mouse
应用	Western Blot,Immunohistochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects mouse Amphiregulin in ELISAs. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human Amphiregulin is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize Amphiregulin-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.3-1.5 µg/mL in the presence of 50 ng/mL Recombinant Mouse Amphiregulin.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	374 (Human); 11839 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism. Authors: Yang Y, Ahn Y, Chen Y, Tan X, Guo L, Gibbons D, Ungewiss C, Peng D, Liu X, Lin S, Thilaganathan N, Wistuba I, Rodriguez-Canales J, McLendon G, Creighton C, Kurie J J Clin Invest, 2014;124(6):2696-708. Species: Mouse Sample Type: Total protein Application: WB Ectodomain shedding of EGFR ligands and TNFR1 dictates hepatocyte apoptosis during fulminant hepatitis in mice. Authors: Murthy A, Defamie V, Smookler DS J. Clin. Invest., 2010;120(8):2731-44. Species: Mouse Sample Type: Whole Cells Application: Neut The Arf-inducing transcription factor Dmp1 encodes a transcriptional activator of amphiregulin, thrombospondin-1, JunB and Egr1. Authors: Mallakin A, Sugiyama T, Kai F, Taneja P, Kendig RD, Frazier DP, Maglic D, Matise LA, Willingham MC, Inoue K Int. J. Cancer, 2010;126(6):1403-16. Species: Mouse Sample Type: Tissue Homogenates Application: WB Pubertal exposure to high fat diet causes mouse strain-dependent alterations in mammary gland development and estrogen responsiveness. Authors: Olson LK, Tan Y, Zhao Y, Aupperlee MD, Haslam SZ Int J Obes (Lond), 2010;34(9):1415-26. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Wounding-induced synthesis of hyaluronic acid in organotypic epidermal cultures requires the release of heparin-binding egf and activation of the EGFR. Authors: Monslow J, Sato N, Mack JA, Maytin EV J. Invest. Dermatol., 2009;129(8):2046-58. Species: Mouse Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse Amphiregulin Ser94-Lys191 Accession # P31955
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Amphiregulin (AR), also known as Schwannoma-derived growth factor (SDGF), is a member of the epidermal growth factor (EGF) family of growth factors which includes, AR, EGF, transforming growth factor-a (TGF-a), heparin binding EGF-like growth factor (HB-EGF), betacellulin (BTC), epiregulin, and the neuregulins-1 through -4. All EGF family members are synthesized as type I transmembrane precursors and contain one or several EGF domains in their extracellular region. The bioactive form of the proteins is released by proteolytic cleavage. The ErbB family of receptors that includes ErbB1‑B4, mediates the biological activities of the EGF family ligands. AR was originally isolated from the conditioned media of PMA-treated MCF-7 human breast carcinoma cell line. AR mRNA expression can be detected in numerous carcinoma cell lines and in the epithelial cells of various human tissues including colon, stomach, breast, ovary and kidney. AR stimulates the proliferation of keratinocytes, mammary epithelial cells, fibroblasts, astrocytes and glial cells. AR is also a growth inhibitor for certain tumor cells. The gene for AR has been mapped to human chromosome 4q13-q21 and mouse chromosome 5. Human and mouse AR cDNA encode 252 and 248 amino acid residue type I membrane proteins, respectively. The two proteins share approximately 69% sequence identity. Mouse AR also shares 81% amino acid sequence homology with rat AR. Several secreted isoforms of AR that vary in length and/or glycosylation level can be found in cell conditioned media. The 98 amino acid residue recombinant AR has better receptor binding and biological activity than the C-terminal truncated forms of the protein.
运输条件	Blue Ice
存放说明	4℃
参考文献	Thompson, S.A. et al. (1996) J. Biol. Chem. 271:17927. Sonoda, H. et al. (1992) Biochem. Biophys. Res. Commun. 185:103. Normanno, N. et al. (2001) Frontiers in Bioscience6:685.

参考图片

Cell Proliferation Induced by Amphiregulin and Neutralization by Mouse Amphiregulin Antibody. Recombinant Mouse Amphiregulin (Catalog # 989‑AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989). The ND₅₀ is typically 0.3-1.5 µg/mL.

Amphiregulin in Mouse Skin. Amphiregulin was detected in immersion fixed frozen sections of mouse skin using Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.