Mouse CCL19/MIP-3 beta Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse CCL19/MIP-3 beta Affinity Purified Polyclonal Ab (25 UG)

货号： AF880-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF880-SP
别名	beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse CCL19/MIP-3 beta in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant rat CCL19/MIP-3 beta is observed and less than 10% cross-reactivity with recombinant human CCL19/MIP‑3 beta is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis inthe BaF3 mouse pro-B cell line transfected with human CCR7. The Neutralization Dose (ND₅₀) is typically 0.8-4 μg/mL in the presence of 50 ng/mL Recombinant Mouse CCL19/MIP‑3 beta.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6363 (Human); 24047 (Mouse); 362506 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow CCR2 plays a critical role in dendritic cell maturation: possible role of CCL2 and NF-kappa B. Authors: Jimenez F, Quinones MP, Martinez HG, Estrada CA, Clark K, Garavito E, Ibarra J, Melby PC, Ahuja SS J. Immunol., 2010;184(10):5571-81. Species: Mouse Sample Type: In Vivo Application: In vivo CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination. Authors: Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN J. Immunol., 2009;182(11):6851-60. Species: Mouse Sample Type: In Vivo Application: In vivo CCR7 modulates pulmonary and lymph node inflammatory responses in cigarette smoke-exposed mice. Authors: Demoor T, Bracke KR, Vermaelen KY, Dupont L, Joos GF, Brusselle GG J. Immunol., 2009;183(12):8186-94. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded CCR7 ligands stimulate the intranodal motility of T lymphocytes in vivo. Authors: Worbs T, Mempel TR, Bolter J, von Andrian UH, Forster R J. Exp. Med., 2007;204(3):489-95. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Defective CCR7 expression on dendritic cells contributes to the development of visceral leishmaniasis. Authors: Ato M, Stager S, Engwerda CR, Kaye PM Nat. Immunol., 2002;3(12):1185-91. Species: Mouse Sample Type: Whole Tissue Application: IHC Fresh/Frozen
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CCL19/MIP-3 beta Gly26-Val107 (Ser108LeuGlu) Accession # Q548P0
内毒素水平	<0.01 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a reported beta chemokine that binds specifically to the chemokine receptor CCR-7/EBI-1/BLR-2. Mouse (human) MIP-3 beta cDNA encodes a 108 (98) amino acid residue precursor protein with a predicted 25 (21) aa residue signal peptide that is cleaved to form the 83 (77) aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T cell zone. The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta has been shown to be chemotactic for T cells and B cells. The MIP-3 beta receptor (CCR7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpes virus 6 or 7.
运输条件	Blue Ice
存放说明	4℃
参考文献	Kim, C.H. et al. (1998) J. Immunol. 160:2418. Ngo, V.N. et al. (1998) J. Exp. Med. 188:181. Rossi, D.L. et al. (1997) J. Immunol. 158:1033. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.

参考图片

Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Mouse CCL19/MIP‑3 beta Antibody. Recombinant Mouse CCL19/MIP‑3 beta (Catalog #
440-M3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP‑3 beta (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880). The ND₅₀ is typically
5-15 µg/mL.

CCL19/MIP‑3 beta in Mouse Splenocytes. CCL19/MIP‑3 beta was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CCL19/MIP‑3 beta in Mouse Dendritic Cells. CCL19/MIP‑3 beta was detected in immersion fixed mouse dendritic cells using Goat Anti-Mouse CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.